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Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

文献类型： 外文期刊

作者： Zhu, Xiaohong 1 ; Taylor, Aaron 2 ; Zhang, Shenyu 1 ; Zhang, Dayong 1 ; Feng, Ying 1 ; Liang, Gaimei 1 ; Zhu, Jian-Kan 1 ;

作者机构： 1.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA

2.Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA

3.Jiangsu Acad Agr Sci, Inst Biotechnol, Nanjing, Jiangsu, Peoples R China

4.Zhejiang Univ, Coll Environm & Resource Sci, Hangzhou, Zhejiang, Peoples R China

5.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Beijing 100864, Peoples R China

关键词： Plant Biology;Issue 91;Aequorin;Case12;abiotic stress;heavy metal stress;copper ion;calcium imaging;Arabidopsis

期刊名称：JOVE-JOURNAL OF VISUALIZED EXPERIMENTS （ 影响因子：1.355； 五年影响因子：1.696 ）

ISSN： 1940-087X

年卷期： 2014 年 91 期

页码：

收录情况： SCI

摘要： Developmental and environmental cues induce Ca2+ fluctuations in plant cells. Stimulus-specific spatial-temporal Ca2+ patterns are sensed by cellular Ca2+ binding proteins that initiate Ca2+ signaling cascades. However, we still know little about how stimulus specific Ca2+ signals are generated. The specificity of a Ca2+ signal may be attributed to the sophisticated regulation of the activities of Ca2+ channels and/or transporters in response to a given stimulus. To identify these cellular components and understand their functions, it is crucial to use systems that allow a sensitive and robust recording of Ca2+ signals at both the tissue and cellular levels. Genetically encoded Ca2+ indicators that are targeted to different cellular compartments have provided a platform for live cell confocal imaging of cellular Ca2+ signals. Here we describe instructions for the use of two Ca2+ detection systems: aequorin based FAS (film adhesive seedlings) luminescence Ca2+ imaging and case12 based live cell confocal fluorescence Ca2+ imaging. Luminescence imaging using the FAS system provides a simple, robust and sensitive detection of spatial and temporal Ca2+ signals at the tissue level, while live cell confocal imaging using Case12 provides simultaneous detection of cytosolic and nuclear Ca2+ signals at a high resolution.

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