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Phage-borne peptidomimetics as immunochemical reagent in dot-immunoassay for mycotoxin zearalenone

文献类型： 外文期刊

作者： He, Qing-hua 1 ; Xu, Yang 1 ; Zhang, Cun-zheng 2 ; Li, Yan-ping 1 ; Huang, Zhi-bing 1 ;

作者机构： 1.Nanchang Univ, Sino German Joint Res Inst, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China

2.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

关键词： Phage;Mycotoxin;Immunoassay;Peptide

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2014 年 39 卷

页码：

收录情况： SCI

摘要： The advantageous characteristics of phage probes and facility of immunoassays were combined to develop a rapid dot-immunoassay for the mycotoxin zearalenone (ZEN). A peptide library of random 12-mers displayed on phage was panned against anti-ZEN antibody. Selected phage-borne peptidomimetics were used as substitute for coating antigen and applied in dot-immunoassay for rapid detecting of ZEN. The binding specificities and reaction kinetics between selected phages and antibody were analyzed by phage ELISA and surface plasmon resonance, respectively. The equilibrium dissociation constant (KD) measured for selected phage (Z5): antibody was 39.8 nM. The cut-off level for this phage-based dot-immunoassay method of detecting ZEN in cereal samples, assessed visually, was 50 mu g kg(-1) and the final results can be obtained within 10 min. The validation of the method was performed by analyzing the spiked samples with ZEN at five levels (15, 30, 45, 60, and 75 mu g kg(-1)) and naturally contaminated cereal samples, the results were in good agreement with the obtained by the commercial ZEN ELISA kit. These results suggest that phage can act as a useful immunochemical reagent in dot-immunoassay for toxic small molecules. (C) 2013 Elsevier Ltd. All rights reserved.

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