您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

NF-kappa B target microRNAs and their target genes in TNF alpha-stimulated HeLa Cells

文献类型： 外文期刊

作者： Zhou, Fei 1 ; Wang, Wei 1 ; Xing, Yujun 1 ; Wang, Tingting 1 ; Xu, Xinhui 1 ; Wang, Jinke 1 ;

作者机构： 1.Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety & Detect, Nanjing 210014, Jiangsu, Peoples R China

关键词： NF-kappa B;miRNA;Target gene;HeLa cell;TNF alpha

期刊名称：BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS （ 影响因子：4.49； 五年影响因子：5.318 ）

ISSN： 1874-9399

年卷期： 2014 年 1839 卷 4 期

页码：

收录情况： SCI

摘要： As a transcription factor, NF-kappa B was demonstrated to regulate the expressions of miRNAs. However, only a few miRNAs have been identified as its targets so far. In this study, by using ChIP-Seq. Genechip and miRNA-Seq techniques, we identified 14 NF-kappa B target miRNAs in TNF alpha-stimulated HeLa Cells, including miR-1276, miR-1286, miR-125b-1-3p, miR-219-1-3p, miR-2467-5p, miR-3200-3p, miR-449c-5p, miR-502-5p, miR-548d-5p, miR-30b-3p, miR-3620-5p, miR-340-3p, miR-4454 and miR-4485. Of these miRNAs, 8 detected miRNAs were also NF-kappa B target misRNAs in TNF alpha-stimulated HepG2 cells. We also identified 16 target genes of 6 miRNAs including miR-125b-1-3p, miR-1286, miR-502-5p, miR-1276, miR-219-1-3p and miR-30b-3p, in TNF alpha-stimulated HeLa cells.Target genes of miR-125b-1-3p and miR-1276 were validated in HeLa and HepG2 cells by transfecting their expression plasmids and mimics. Bioinformatic analysis revealed that two potential target genes of miR-1276, BMP2 and CASP9, were enriched in disease phenotypes. The former is enriched in osteoarthritis, and the latter is enriched in Type 2 diabetes and lung cancer, respectively. These findings suggested that this little known miRNA might play roles in these diseases via its two target genes of BMP2 and CASP9. The expression of miR-125b-1 regulated by NF-kappa B has been reported in diverse cell types under various stimuli, this study found that its expression was also significantly regulated by NF-kappa B in TNF alpha-stimulated HeLa and HepG2 cells. Therefore, this miRNA was proposed as a central mediator of NF-kappa B pathway. These findings provide new insights into the functions of NF-kappa B in its target miRNA-related biological processes and the mechanisms underlying the regulation of these miRNAs. (C) 2014 Elsevier B.V. All rights reserved.

  • 相关文献

[1]Identification of novel and salt-responsive miRNAs to explore miRNA-mediated regulatory network of salt stress response in radish (Raphanus sativus L.). Sun, Xiaochuan,Xu, Liang,Wang, Yan,Yu, Rugang,Luo, Xiaobo,Gong, Yiqin,Wang, Ronghua,Limera, Cecilia,Liu, Liwang,Sun, Xiaochuan,Xu, Liang,Luo, Xiaobo,Zhu, Xianwen,Zhang, Keyun. 2015

[2]Molecular cloning of the rabbit interleukin 6 promoter: Functional characterization of rabbit hemorrhagic disease virus response elements in RK-13 cells. Liu, Xing,Hu, Bo,Wang, Fang,Song, Yanhua,Fan, Zhiyu,Wei, Houjun,Qiu, Rulong,Xu, Weizhong.

[3]畜禽肌内脂肪沉积的基因调控及其作用机制. 汪凯歌,闫俊书,宦海琳. 2024

[4]陆地棉胚珠及纤维发育过程中miRNA分离与鉴定. 王芹芹,陈旭生,Pieter Bas Kwak,邱承祥,刘飞,马晓杰,杨志敏. 2010

[5]两品种鸭下丘脑miRNA的鉴定及其表达验证分析. 陈蓉,应诗家,陈哲,于建宁. 2017

[6]鸡p53抑制传染性法氏囊病病毒复制及gga-miR-2127对其调控的分子机制. 欧阳伟,王永山,孟凯,潘群兴,王晓丽,夏兴霞,诸玉梅,毕振威,董晨红. 2016

[7]gga-miR-9*对传染性法氏囊病病毒复制的影响. 欧阳伟,王永山,杜希宁,刘华洁,张海彬. 2014

[8]gga-miR-21过表达细胞株对传染性法氏囊病病毒复制的影响. 欧阳伟,朱向东,王永山,王永强,潘群兴,毕振威,王笑梅. 2012

[9]RNA分子在植物韧皮部长距离运输的研究进展. 李苹芳,羊杏平,徐锦华,刘广,姚协丰,高长洲,朱凌丽. 2013

[10]gga-miR-21过表达细胞株对传染性法氏囊病病毒复制的影响. 朱向东,王永山,王永强,潘群兴,毕振威,王笑梅. 2013

[11]山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析. 李基棕,李文良,毛立,郝飞,杨蕾蕾,张纹纹,江杰元. 2017

[12]乙型脑炎病毒感染猪睾丸细胞的miRNA表达谱差异分析. 张元鹏,杨占娜,杨利,李兰,于晓明,杜露平,陈瑾,侯立婷,乔绪稳,侯继波,郑其升. 2019

[13]金星无核与其短节间突变体紫金早生的节间长度差异机制初探. 王博,王壮伟,王西成,钱亚明,吴伟民. 2021

[14]基于比较转录组学分析过量表达miRNA玉米耐旱性差异机制. 柴文波,李淑芬,祝庆,许瀚元,李洪涛,袁超,王军. 2024

[15]Effects of duloxetine on microRNA expression profile in frontal lobe and hippocampus in a mouse model of depression. Pan, Bing,Liu, Yamei. 2015

[16]Dual transcriptome analysis reveals insights into the response to Rice black-streaked dwarf virus in maize. Zhou, Yu,Duan, Canxing,Hao, Zhuanfang,Li, Mingshun,Yong, Hongjun,Zhang, Degui,Zhang, Shihuang,Weng, Jianfeng,Li, Xinhai,Zhou, Yu,Xu, Zhennan,Wang, Zhenhua,Chen, Yanping,Meng, Qingchang,Wu, Jirong.

[17]Conserved miRNAs and Their Response to Salt Stress in Wild Eggplant Solanum linnaeanum Roots. Zhuang, Yong,Zhou, Xiao-Hui,Liu, Jun. 2014

[18]High throughput sequencing of herbaceous peony small RNAs to screen thermo-tolerance related microRNAs. Hao, Zhaojun,Liu, Ding,Hao, Zhaojun,Liu, Ding,Gong, Saijie,Zhao, Daqiu,Tao, Jun,Zhao, Daqiu,Tao, Jun.

[19]gga-miR-9*inhibits IFN production in antiviral innate immunity by targeting interferon regulatory factor 2 to promote IBDV replication. Ouyang, Wei,Liu, Hua-jie,Zhang, Hai-bin,Ouyang, Wei,Wang, Yong-shan,Du, Xi-ning,Liu, Hua-jie,Ouyang, Wei,Wang, Yong-shan.

[20]gga-miR-2127 downregulates the translation of chicken p53 and attenuates chp53-mediated innate immune response against IBDV infection. Ouyang, Wei,Wang, Yong-shan,Meng, Kai,Pan, Qun-xing,Wang, Xiao-li,Xia, Xing-xia,Zhu, Yu-mei,Bi, Zhen-wei,Zhang, Hai-bin,Ouyang, Wei,Wang, Yong-shan,Meng, Kai,Pan, Qun-xing,Wang, Xiao-li,Xia, Xing-xia,Zhu, Yu-mei,Bi, Zhen-wei,Ouyang, Wei,Luo, Kai,Zhang, Hai-bin.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台