UV-Initiated Copolymerization Route for Facile Fabrication of Epoxy-Functionalized Micro-Zone Plates
文献类型： 外文期刊
作者： Li, Li 1 ; Bi, Xiao-Dong 2 ; Feng, Min 1 ; Zhu, Jia-Ting 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Facil & Equipment Agr, Dept Nucl Agr Sci, Nanjing 210014, Peoples R China
2.Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci, Nanjing 210093, Jiangsu, Peoples R China
关键词： applications;biomaterials;copolymers
期刊名称：JOURNAL OF APPLIED POLYMER SCIENCE （ 影响因子：3.125； 五年影响因子：2.754 ）
ISSN： 0021-8995
年卷期： 2014 年 131 卷 2 期
页码：
收录情况： SCI
摘要： Bisphenol A-based epoxy acrylate (BABEA), a commercial ultraviolet (UV)-curable material, was introduced as a new manufacturing material for facile fabrication of epoxy-functionalized micro-zone plates through UV-initiated copolymerization using glycidyl methacrylate (GMA) as the functional monomer. The poly (BABEA-co-GMA) was highly transparent in visible range while highly opaque when the wavelength is less than 295 nm, and of high replication fidelity. X-ray photoelectron spectroscope (XPS) results indicated the existence of epoxy groups on the surface of the poly (BABEA-co-GMA), which allowed for binding protein through an epoxy-amino group reaction. A fabrication procedure was proposed for manufacturing BABEA based epoxy-functionalized micro-zone plates. The fabrication procedure was very simple; obviating the need of micromachining equipments, wet etching or imprinting techniques. To evaluate the BABEA-based epoxy-functionalized micro-zone plates, alpha-fetoprotein (AFP) was immobilized onto the capture zone for chemiluminescent (CL) detection in a noncompetitive immune response format. The proposed AFP immunoaffinity micro-zone plate was demonstrated as a low cost, flexible, homogeneous, and stable assay for alpha-fetoprotein (AFP). (C) 2013 Wiley Periodicals, Inc.
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