Genetic purity testing of F-1 hybrid seed with molecular markers in cabbage (Brassica oleracea var. capitata)
文献类型： 外文期刊
作者： Ye, Shan 1 ; Wang, Yan 1 ; Huang, Danqiong 2 ; Li, Jianbin 3 ; Gong, Yiqin 1 ; Xu, Liang 1 ; Liu, Liwang 1 ;
作者机构： 1.Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Engn Res Ctr Hort Crop Germplasm Enhancement & Ut, Minist Educ PR China,Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
2.N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA
3.Jiangs
关键词： Cabbage;Seed genetic purity;DNA molecular markers;Hybrid cultivar;Grow-out-trials (GOTs)
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
ISSN： 0304-4238
年卷期： 2013 年 155 卷
页码：
收录情况： SCI
摘要： The seed genetic purity of cabbage F-1 hybrid cultivar, 'Sugan 21', was analyzed with PCR-based molecular markers including SSR, RAPD, ISSR and SRAP in this study. Among the total of 325 primers including 85 SSR, 80 RAPD, 96 ISSR and 64 SRAP screened, only seven primers could produce both female parent-specific (FPS) and male parent-specific (MPS) markers. A total of 216 F-1 hybrid individuals were genotyped with these seven primers belonging to three molecular marker systems. It was found that four out of the 216 F-1 individuals were false hybrids, among which three could only generate FPS markers and the rest generated MPS markers only. Therefore, the genetic purity of 'Sugan 21' seed lot used in this study was calculated as 98.15%. Grow-out-trials (GOTs) were carried out to test the seed genetic purity and validate the authenticity of the molecular markers analysis, and it was found that five out of the 216 F-1 plants were false hybrids and the seed purity from GOTs was 97.69%. These results indicated that the molecular markers could be used as a more rapid, practical and efficient tool in quality control of the cabbage commercial hybrid seeds. (C) 2013 Elsevier B.V. All rights reserved.
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