Development of SSRs Based on the Whole Genome and Screening of Bolting-Resistant SSR Marker in Brassica oleracea L.
文献类型： 外文期刊
作者： Zhao, Tong 1 ; Miao, Liming 1 ; Zou, Minghua 1 ; Hussain, Iqbal 1 ; Yu, Hongrui 1 ; Li, Jia 1 ; Sun, Nairan 1 ; Kong, Lijun 1 ; Wang, Shenyun 4 ; Li, Jianbin 4 ; Yu, Xiaolin 1 ;
作者机构： 1.Zhejiang Univ, Dept Hort, 866 Yuhangtang Rd, Hangzhou 310058, Peoples R China
2.Zhejiang Prov Key Lab Hort Plant Integrat Biol, Hangzhou 310058, Peoples R China
3.Zhejiang Univ, Grp Vegetable Breeding, Hainan Inst, Sanya 572025, Peoples R China
4.Jiangsu Acad Agr Sci, Inst Vegetable Sci, Nanjing 210014, Peoples R China
关键词： Brassica oleracea L.; cabbage; SSR identification; molecular makers; genetic diversity; bolting
期刊名称：HORTICULTURAE （ 影响因子：3.1； 五年影响因子：3.4 ）
ISSN：
年卷期： 2024 年 10 卷 5 期
页码：
收录情况： SCI
摘要： Simple sequence repeats (SSRs), also known as microsatellites, stand out as the most crucial molecular markers in both animals and plants owing to their high polymorphism, extensive information content, ease of detection through polymerase chain reaction (PCR) assays, and widespread distribution across the genome. In this study, a total of 125,443 SSR loci were identified from the whole-genome sequence of B. oleracea, and 82,948 primer pairs for SSR have been designed. Furthermore, each primer pair is designated with a unique identifier (ranging from BolSSR00001 to BolSSR82984). Our findings indicated that certain markers within them could be transferred to other cruciferous crops. In addition, a total of 336 pairs of SSR primers have been used to screen the polymorphism between the bolting-resistant and bolting-easy gene pools. After the test of verification with F2 generation individual plants, we obtained an SSR dominant marker, BolSSR040196, linked with bolting-resistant locus in cabbage, and the genetic distance between this SSR marker and the bolting-resistant locus was 10.69 cM. Moreover, BolSSR040196 is located on the C05 chromosome with a CT motif, characterized by a repeat of 9 in bolting-easy plants and 11 in bolting-resistant plants. Haplotype analysis showed that the correct prediction rate reached 82.35%. The BolSSR040196 marker can be used in marker-assisted selection (MAS) breeding, offering a straightforward and efficient approach for bolting-resistant cabbage breeding in the future.
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