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Effect of different selenium sources and levels on porcine circovirus type 2 replication in vitro

文献类型： 外文期刊

作者： Pan, Qunxing 1 ; Huang, Kehe 1 ; He, Kongwang 2 ; Lu, Fuzeng 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu Prov, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Peoples R China

关键词： porcine circovirus type 2;sodium selenite;kappa-selenocarrageenan;DL-selenomethionine;real-time PCR

期刊名称：JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY （ 影响因子：3.849； 五年影响因子：4.252 ）

ISSN： 0946-672X

年卷期： 2008 年 22 卷 2 期

页码：

收录情况： SCI

摘要： Porcine circovirus type 2 (PCV2) has been linked to several disease syndromes during the last decade. A deficiency in selenium has also been associated with the increases of virulence of some viruses and severity of infectious disease. In order to evaluate the effect of different selenium sources and levels on PCV2 replication in PK-15 cells, three selenium Sources, i.e. sodium selenite, kappa-selenocarrageenan and DL-Selenomethionine at. concentrations of 0, 2, 4, 8, and 16 mu mol/L were used throughout this experiment. PCV2 loads in PK-15 cells were measured by a newly developed real-time quantitative PCR. A significantly inhibitive effect of DL-Selenomethionine on PCV2 replication in vitro was demonstrated and the inhibition was concentration dependent within the range of 2-16 mu mol/L. The inhibitive effect of DL-selenomethionine on PCV2 replication may be caused by enhanced activity of glutathione peroxidase. Out results may serve as a basis for further studies of the biological function of selenium and control of PCV2 infection. (C) 2008 Elsevier GmbH. All rights reserved.

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