您好，欢迎访问河北省农林科学院 机构知识库！
河北省农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2

文献类型： 外文期刊

作者： Guo, Qinggang 1 ; Li, Shezeng 1 ; Lu, Xiuyun 1 ; Li, Baoqing 1 ; Ma, Ping 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Integrated Pest Management Ctr Hebei Prov, Baoding 071000, Hebei, Peoples R China

关键词： Bacillus subtilis;antagonism;Verticillium dahliae;mini-Tn10

期刊名称：GENETICS AND MOLECULAR BIOLOGY （ 影响因子：1.771； 五年影响因子：2.584 ）

ISSN： 1415-4757

年卷期： 2010 年 33 卷 2 期

页码：

收录情况： SCI

摘要： The Bacillus subtilis strain NCD-2 is an important biocontrol agent against cotton verticillium wilt and cotton sore shin in the field, which are caused by Verticillium dahliae Kleb and Rhizoctonia solani Kuhn, respectively. A mutant of strain NCD-2, designated M216, with decreased antagonism to V. dahliae and R. solani, was selected by mini-Tn10 mutagenesis and in vitro virulence screening. The inserted gene in the mutant was cloned and identified as the phoR gene, which encodes a sensor kinase in the PhoP/PhoR two-component system. Compared to the wild-type strain, the APase activities of the mutant was decreased significantly when cultured in low phosphate medium, but no obvious difference was observed when cultured in high phosphate medium. The mutant also grew more slowly on organic phosphate agar and lost its phosphatidylcholine-solubilizing ability. The suppression of cotton seedling damping-off in vivo and colonization of the rhizosphere of cotton also decreased in the mutant strain when compared with the wild type strain. All of these characteristics could be partially restored by complementation of the phoR gene in the M216 mutant.

  • 相关文献

[1]Effects of Cry1 Ac toxin of Bacillus thuringiensis and nuclear polyhedrosis virus of Helicoverpa armigera (Hubner) (Lepidoptera : Noctuidae) on larval mortality and pupation. Liu, Xiaoxia,Zhang, Qingwen,Xu, Baoliang,Li, Jiancheng. 2006

[2]A novel Gossypium barbadense ERF transcription factor, GbERFb, regulation host response and resistance to Verticillium dahliae in tobacco. Liu, Jianguang,Wang, Yongqiang,Zhao, Guiyuan,Zhao, Junli,Du, Haiying,Zhang, Hanshuang,He, Xiaoliang.

[3]The PhoR/PhoP two-component system regulates fengycin production in Bacillus subtilis NCD-2 under low-phosphate conditions. Guo Qing-gang,Dong Li-hong,Wang Pei-pei,Li She-zeng,Zhao Wei-song,Lu Xiu-yun,Zhang Xiao-yun,Ma Ping. 2018

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：河北省农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台