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Reasons for the differences in biotransformation of conjugated linoleic acid by Lactobacillus plantarum

文献类型： 外文期刊

作者： Liu, Xin-Xin 1 ; Zhang, Hong-Yun 1 ; Song, Xin 1 ; Yang, Ying 2 ; Xiong, Zhi-Qiang 1 ; Xia, Yong-Jun 1 ; Ai, Lian-Zhon 1 ;

作者机构： 1.Univ Shanghai Sci & Technol, Shanghai Engn Res Ctr Food Microbiol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Peoples R China

关键词： conjugated linoleic acid (CLA); Lactobacillus plantarum; biotransformation; gene transcription

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.034； 五年影响因子：4.354 ）

ISSN： 0022-0302

年卷期： 2021 年 104 卷 11 期

页码：

收录情况： SCI

摘要： Conjugated linoleic acid (CLA) has attracted a great deal of attention for its functions in weight loss, regula-tion of metabolism, and antioxidant capabilities. Many microorganisms, including rumen bacteria, propionic acid bacilli, and Lactobacillus, have CLA biotransforma-tion ability. The CLA production capability of different species is different, as are those different strains of the same species. However, the reasons for this discrepancy remain unclear. In this study, 14 strains of Lactobacillus plantarum were found, through gas chromatography-mass spectrometry analysis, to be capable of convert-ing linoleic acid to CLA. The transcriptional levels of CLA-related genes in the high-(AR195, WCFS1, and AR488) and low-yield strains (AR176, AR269, and AR611) were analyzed using real-time quantita-tive PCR. The transcriptional levels of cla-hy, cla-dh, and cla-dc in AR195 were the lowest in the exponential phase, but it had the highest CLA yield. Correlation analysis showed no correlation between CLA yield and the transcription level of these genes in the exponential phase. The results showed that a high transcriptional level in the exponential phase of cla-hy, cla-dh, and cla-dc did not necessarily lead to high CLA produc-tion. Investigation of the transcription level in different growth phases showed that the CLA biotransformation abilities of Lactobacillus plantarum strains significantly depended on the transcriptional maintenance of cla-hy, cla-dh, and cla-dc. We observed a correlation between CLA production and increased levels of cla-hy tran-scription, but a prerequisite is needed: the transcription of cla-dh and cla-dc should be upregulated and main-tained a high transcriptional level during the platform period. This study provides a new strategy for screening high CLA-producing strains. It also lays a theoretical foundation for regulating CLA biotransformation and increasing the yield of CLA.

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