Pre-column derivatization with trimethylsilyl diazomethane coupled with ASE-SPE-GC-MS/MS method for the quantification and validation of penicillin G residues in poultry tissues and pork
文献类型： 外文期刊
作者： Liu, Chujun 1 ; Xie, Xing 2 ; Guo, Yawen 1 ; Wang, Bo 3 ; Xie, Kaizhou 1 ; Dong, Yuhao 4 ; Yang, Chenggen 5 ; Feng, Zhixin 2 ; Bao, Wenbin 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing 210014, Peoples R China
3.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China
4.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
5.Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225009, Peoples R China
6.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China
关键词： Penicillin G; TMSD; ASE; Poultry tissues; Pork; GC-MS; MS; Food analysis; Food composition
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.52； 五年影响因子：4.942 ）
ISSN： 0889-1575
年卷期： 2023 年 115 卷
页码：
收录情况： SCI
摘要： A novel, simple and high-sensitivity gas chromatography-tandem mass spectrometry (GC-MS/MS) method combined with external calibration was developed for the qualitative and quantitative determination of peni-cillin G residues in poultry tissues and pork. Samples were extracted by an ASE 350 apparatus with 0.2 mol/L phosphate buffer solution (pH 8.0) and purified on HLB solid-phase extraction (SPE) cartridges (60 mg/3 mL). Trimethylsilyl diazomethane (TMSD) was selected as the derivatization reagent for precolumn derivatization. The recoveries of analytes at the limit of quantitation (LOQ) and 25, 50 and 100 mu g/kg ranged from 80.67% to 96.18%, with RSDs below 3.17%. The determination coefficient (R2) values were above 0.9993 in the corre-sponding concentration range. The limits of detection (LODs) (S/N & GE; 3) and LOQs (S/N & GE; 10) ranged from 1.50 to 4.10 mu g/kg and 4.50-8.20 mu g/kg, respectively. In addition, the newly established method was implemented to analyze poultry tissues and pork purchased from a local market, demonstrating the sensitivity and applicability of the method.
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