Pre-column derivatization with trimethylsilyl diazomethane coupled with ASE-SPE-GC-MS/MS method for the quantification and validation of penicillin G residues in poultry tissues and pork
文献类型： 外文期刊
作者： Liu, Chujun 1 ; Xie, Xing 2 ; Guo, Yawen 1 ; Wang, Bo 3 ; Xie, Kaizhou 1 ; Dong, Yuhao 4 ; Yang, Chenggen 5 ; Feng, Zhixin 2 ; Bao, Wenbin 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Joint Int Res Lab Agr & Agriprod Safety, Yangzhou 225009, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr, Nanjing 210014, Peoples R China
3.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China
4.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China
5.Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225009, Peoples R China
6.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China
关键词： Penicillin G; TMSD; ASE; Poultry tissues; Pork; GC-MS; MS; Food analysis; Food composition
期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.52； 五年影响因子：4.942 ）
ISSN： 0889-1575
年卷期： 2023 年 115 卷
页码：
收录情况： SCI
摘要： A novel, simple and high-sensitivity gas chromatography-tandem mass spectrometry (GC-MS/MS) method combined with external calibration was developed for the qualitative and quantitative determination of peni-cillin G residues in poultry tissues and pork. Samples were extracted by an ASE 350 apparatus with 0.2 mol/L phosphate buffer solution (pH 8.0) and purified on HLB solid-phase extraction (SPE) cartridges (60 mg/3 mL). Trimethylsilyl diazomethane (TMSD) was selected as the derivatization reagent for precolumn derivatization. The recoveries of analytes at the limit of quantitation (LOQ) and 25, 50 and 100 mu g/kg ranged from 80.67% to 96.18%, with RSDs below 3.17%. The determination coefficient (R2) values were above 0.9993 in the corre-sponding concentration range. The limits of detection (LODs) (S/N & GE; 3) and LOQs (S/N & GE; 10) ranged from 1.50 to 4.10 mu g/kg and 4.50-8.20 mu g/kg, respectively. In addition, the newly established method was implemented to analyze poultry tissues and pork purchased from a local market, demonstrating the sensitivity and applicability of the method.
- 相关文献
作者其他论文 更多>>
-
Identification of the Effects of 5-Azacytidine on Porcine Circovirus Type 2 Replication in Porcine Kidney Cells
作者：Shan, Yiyi;Wu, Shenglong;Bao, Wenbin;Wu, Zhengchang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Xiao, Qi;He, Kongwang;Wu, Zhengchang
关键词：5-Azacytidine; porcine circovirus type 2; porcine kidney cells
-
Combining GC-MS/MS with a LLE-SPE sample pretreatment step to simultaneously analyse enrofloxacin and ofloxacin residues in chicken tissues and pork
作者：Pang, Maoda;Wang, Ran;Xu, Junjie;Tang, Yayun;Guo, Yawen;Ding, Hao;Zhang, Tao;Zhang, Genxi;Guo, Xiaodong;Dai, Guojun;Xie, Kaizhou;Xie, Xing;Xie, Xing
关键词：Chicken tissues; Pork; Enrofloxacin; Ofloxacin; GC-MS/MS
-
Enhancing Point-of-Care Diagnosis of African Swine Fever Virus (ASFV) DNA with the CRISPR-Cas12a-Assisted Triplex Amplified Assay
作者：Zhu, Dan;Su, Tong;Sun, Tao;Qin, Xingcai;Su, Shao;Chao, Jie;Wang, Lianhui;Zhu, Dan;Su, Tong;Sun, Tao;Qin, Xingcai;Su, Shao;Chao, Jie;Wang, Lianhui;Li, Fang;Zhao, Dongming;Bai, Yun;Shao, Guoqing;Feng, Zhixin
关键词：
-
Regulatory Effects of Diverse DSF Family Quorum-Sensing Signals in Plant-Associated Bacteria
作者：Li, Kaihuai;Ma, Chaoyun;Zhou, Xue;Xiong, Chunlan;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Ma, Chaoyun;Zhou, Xue;Xiong, Chunlan;Wang, Yong;Liu, Fengquan;Li, Kaihuai;Wang, Yong;Wang, Bo;Liu, Fengquan
关键词：bacterial intraspecies regulation; diffusible signal factor (DSF); plant innate immunity; quorum sensing (QS)
-
Development of a QuEChERS-HPLC-FLD Procedure for the Simultaneous Detection of Residues of Florfenicol, Its Metabolite Florfenicol Amine, and Three Fluoroquinolones in Eggs
作者：Guo, Yawen;Hong, Lu;Liu, Shuyu;Zhu, Yali;Zhang, Genxi;Xie, Kaizhou;Guo, Yawen;Hong, Lu;Liu, Shuyu;Zhu, Yali;Zhang, Genxi;Xie, Kaizhou;Gao, Pengfei;Gao, Pengfei;Xie, Xing
关键词：florfenicol; fluoroquinolones; HPLC-FLD; QuEChERS; egg
-
Determination of Tilmicosin in Poultry and Pork Meat by a Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) Preparation Method and Gas Chromatography-Tandem Mass Spectrometry (GC-MS/MS)
作者：Guo, Yawen;Liu, Shuyu;Tang, Yayun;Zhu, Yali;Xie, Kaizhou;Guo, Yawen;Liu, Shuyu;Tang, Yayun;Zhu, Yali;Xie, Kaizhou;Xie, Xing;Xie, Xing
关键词：Food safety; gas chromatography-tandem mass spectrometry (GC-MS/MS); poultry and pork meat; QuEChERS; tilmicosin
-
Aqueous M. oleifera leaf extract alleviates DSS-induced colitis in mice through suppression of inflammation
作者：Zhang, Shuai;Cao, Yanan;Huang, Yanjie;Zhang, Shuoshuo;Wang, Guangzheng;Bao, Wenbin;Fang, Xiaomin;Bao, Wenbin
关键词：M. oleifera; Inflammatory bowel disease; Intestinal organoids; TNF-& alpha; Dextran sulfate sodium; Network pharmacology
