Rabbit hemorrhagic disease virus poly(A) tail is not essential for the infectivity of the virus and can be restored in vivo
文献类型： 外文期刊
作者： Liu, G. -Q. 1 ; Ni, Z. 1 ; Yun, T. 1 ; Yu, B. 1 ; Zhu, J. -M. 1 ; Hua, J. -G. 1 ; Chen, J. -P. 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2008 年 153 卷 5 期
页码：
收录情况： SCI
摘要： The precise role of the poly(A) tail at the 3'-end of the calicivirus RNA genome is unknown. To study the relationship between the presence of the poly(A) tail and the infectivity and replication of rabbit hemorrhagic disease virus (RHDV), mutants of an infectious cDNA clone of RHDV were constructed, and RK13 cells were transfected with transcripts from these mutants. Transcripts with and without a poly(A) had a fairly similar ability to infect and replicate, suggesting that a long 3'-terminal poly(A) is not essential for infectivity and replication. RT-PCR with specific primers, using viral RNA recovered from RK13 cells transfected with poly(A)-deficient RNA transcripts, showed that the poly(A) tail was restored in vivo.
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