Duplex SYBR Green I real-time RT-PCR for Simultaneous Detection of Goose Astrovirus Genotypes 1 and 2
文献类型： 外文期刊
作者： Zhai, Qi 1 ; Zhao, Liang 1 ; Jia, Chun-Ling 1 ; Zhou, Xiu-Rong 1 ; Luo, Sheng-Jun 1 ; Wen, Xiao-Hui 1 ; Lv, Dian-Hong 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Anim Hlth, Guangzhou 510640, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Prevent & Control Avian Influenza & Other, Guangzhou 510640, Peoples R China
3.Key Lab Livestock Dis Prevent Guangdong Prov, Guangzhou 510640, Peoples R China
4.Minist Agr & Rural Affairs, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou 510640, Peoples R China
关键词： Goose astrovirus genotype 1 and 2 (GAstV 1 and GAstV 2); Duplex SYBR Green I realtime RT-PCR (qRT-PCR); Diagnostic tool; Waterfowl farming
期刊名称：PAKISTAN VETERINARY JOURNAL （ 影响因子：2.3； 五年影响因子：1.5 ）
ISSN： 0253-8318
年卷期： 2023 年 43 卷 4 期
页码：
收录情况： SCI
摘要： As the prevalence and complexity of goose astrovirus genotype 1 and 2 (GAstV 1 and GAstV 2) infections continue to rise in China's goose farming industry, a rapid, reliable and cost-effective diagnostic tool is urgently needed. Duplex SYBR Green I real-time RT-PCR (qRT-PCR) method was developed, which is capable of simultaneous detection of GAstV 1 and GAstV 2. We designed two primer pairs, targeting the viruses conserved genomic regions, facilitating the distinction of GAstV 1 and GAstV 2 through unique melting curve peaks. This method exhibited strong linear relationships in standard curves (R2 = 0.99), and detection limits were 3.18 x 102 and 3.16 x 102 copies/mu L for GAstV 1 and GAstV 2, respectively. With intra- and inter-assay variation below 2%, the assay exhibits excellent repeatability. This duplex qRT-PCR, displaying consistent detection rates comparable to a TaqMan-based method, offers a promising, rapid, and cost-effective diagnostic strategy for waterfowl farming.
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