您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Coexistence of multiple genotypes of porcine epidemic diarrhea virus with novel mutant S genes in the Hubei Province of China in 2016

文献类型： 外文期刊

作者： Zeng, Zhe 1 ; Li, Ting-Ting 2 ; Jin, Xin 2 ; Peng, Fu-Hu 2 ; Song, Nian-Hua 2 ; Peng, Gui-Qing 1 ; Ge, Xing-Yi 3 ;

作者机构： 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China

2.Hubei Anim Dis Prevent & Control Ctr, Wuhan 430070, Hubei, Peoples R China

3.Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China

关键词： porcine epidemic diarrhea virus (PEDV);coronavirus;spike;genetic variation

期刊名称：VIROLOGICA SINICA （ 影响因子：4.327； 五年影响因子：4.08 ）

ISSN： 1674-0769

年卷期： 2017 年 32 卷 4 期

页码：

收录情况： SCI

摘要： The emergence of highly virulent porcine epidemic diarrhea virus (PEDV) variants in China caused huge economic losses in 2010. Since then, large-scale sporadic outbreaks of PED caused by PEDV variants have occasionally occurred in China. However, the molecular diversity and epidemiology of PEDV in different provinces has not been completely understood. To determine the molecular diversity of PEDV in the Hubei Province of China, we collected 172 PED samples from 34 farms across the province in 2016 and performed reverse transcription polymerase chain reaction (RT-PCR) by targeting the nucleocapsid (N) gene. Seventy-four samples were found to be PEDV-positive. We further characterized the complete spike (S) glycoprotein genes from the positive samples and found 21 different S genes with amino acid mutations. The PEDV isolates here presented most of the genotypes which were found previously in field isolates in East and South-East Asia, North America, and Europe. Besides the typical Genotypes I and II, the INDEX groups were also found. Importantly, 58 new amino acids mutant sites in the S genes, including 44 sites in S1 and 14 sites in S2, were first described. Our results revealed that the S genes of PEDV showed variation and that diverse genotypes of PEDV coexisted and were responsible for the PED outbreaks in Hubei in 2016. This work highlighted the complexity of the epidemiology of PEDV and emphasized the need for reassessing the efficacy of classic PEDV vaccines against emerging variant strains and developing new vaccines to facilitate the prevention and control of PEDV in fields.

  • 相关文献

[1]Population Genetic Analyses of Fusarium asiaticum Populations from Barley Suggest a Recent Shift Favoring 3ADON Producers in Southern China. Zhang, Z.,Chen, W. Q.,Xu, J.,Xu, J. S.,Feng, J.,van der Lee, T.,Waalwijk, C.,Yang, L.,Yu, D.. 2010

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台