您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

An RNA-Seq screen of the dithiothreitol-induced apoptosis response in chicken cardiomyocytes

文献类型： 外文期刊

作者： Wan, Chunyun 1 ; Xiang, Jinmei 2 ; Guo, Rui 4 ; Yang, Shijin 2 ; Li, Youwen 2 ; Guo, Dingzong 2 ;

作者机构： 1.Yangtze Univ, Coll Anim Sci, Jingzhou, Hubei, Peoples R China

2.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China

3.Hubei Vocat Coll Biotechnol, Dept Anim Sci, Wuhan, Hubei, Peoples R China

4.Hubei Acad Agr Sci, Hubei Key Lab Embryo & Mol Breeding, Wuhan, Hubei, Peoples R China

关键词： RNA-Seq;apoptosis;cardiomyocytes;apoptosis inducer;DTT

期刊名称：INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY （ 影响因子：0.252； 五年影响因子：1.188 ）

ISSN： 1936-2625

年卷期： 2017 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Cardiomyocytes in postnatal heart are terminally differentiated and thus possess limited capacity to regenerate. Loss of cardiomyocytes through apoptosis and necrosis can lead to heart failure and other serious consequences. Compared to mammals, substantially less research has been conducted on chicken cardiomyocytes; thus, the apoptosis pathways and many relevant genes remain unknown. To elucidate the pathways and genes involved in chicken myocardial cell apoptosis, primary cultures of chicken embryo cardiomyocytes were sham-treated or exposed to the apoptosis inducer dithiothreitol (DTT), and RNA-Seq with technical replicates conducted to identify differentially expressed genes and transcripts. Relevant differentially expressed transcripts were used to construct a protein-protein interactive network for chicken apoptosis. Sequencing detected a total of 19,268 known genes and 2,160 novel genes. In the DTT treatment group, 6468 genes showed significant differential expression based on DEseq analysis, of which 47.99% were upregulated and 52.01% downregulated. The initiation of apoptosis was primarily dependent on caspase-8 and caspase-9, whereas the execution stage was dependent on caspase-3. Up-regulated genes also included many involved in the WNT and MAPK pathways. Repression of apoptosis was primarily dependent on Bcl-2, IAP, and SIVA. These results provide a foundation for detailed analysis of avian myocardial apoptosis, including genes and LncRNAs that constitute, regulate, or link various pro-and anti-apoptotic pathways.

  • 相关文献

[1]高低产蛋鸡产蛋后期卵巢组织差异表达基因筛选及其功能分析. 陈林,王家乡,吴艳,皮劲松,蒲跃进. 2024

[2]利用转录组技术鉴定影响硒都黑猪肌内脂肪含量性状关键基因及标记的研究. 吴俊静,田海斌,刘晓哲,乔木,周佳伟,孙华,彭先文,梅书棋. 2023

[3]Transcriptome Analyses Shed New Insights into Primary Metabolism and Regulation of Blumeria graminis f. sp tritici during Conidiation. Zeng, Fan-Song,Yang, Li-Jun,Yu, Da-Zhao,Zeng, Fan-Song,Xue, Min-Feng,Zhang, Xue-Jiang,Gong, Shuang-Jun,Yang, Li-Jun,Shi, Wen-Qi,Yu, Da-Zhao,Zeng, Fan-Song,Xue, Min-Feng,Zhang, Xue-Jiang,Gong, Shuang-Jun,Yang, Li-Jun,Shi, Wen-Qi,Yu, Da-Zhao,Menardo, Fabrizio. 2017

[4]The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of Zoonotic Streptococcus suis. Zhang, Chunyan,Sun, Wen,Dong, Mengmeng,Liu, Wanquan,Li, Lu,Xu, Zhuofei,Zhou, Rui,Tan, Meifang,Gao, Ting,Li, Lu,Xu, Zhuofei,Zhou, Rui. 2017

[5]Breed-specific transcriptome response of spleen from six to eight week old piglet after infection with Streptococcus suis type 2. Gaur, U.,Luo, Q. P.,Yuan, F. Y.,Wu, H. Y.,Qiao, M.,Mei, S. Q.,Liu, G. S.,Gaur, U.,Luo, Q. P.,Yuan, F. Y.,Wu, H. Y.,Qiao, M.,Mei, S. Q.,Liu, G. S.,Xiong, Y. Y.,Wimmers, K.,Li, K..

[6]Lentivirus-mediated Sirt1 shRNA and resveratrol independently induce porcine preadipocyte apoptosis by canonical apoptotic pathway. Pang, Wei-Jun,Xiong, Yan,Zhang, Zhao,Wei, Ning,Yang, Gong-she,Chen, Ni.

[7]Acylhydrazone derivatives as potential anticancer agents: Synthesis, bio-evaluation and mechanism of action. Yu, Xifang,Shi, Liqiao,Ke, Shaoyong.

[8]Synthesis, antitumor activity and mechanism of action of novel 1,3-thiazole derivatives containing hydrazide-hydrazone and carboxamide moiety. He, Haifeng,Wang, Xiaoyan,He, Hongwu,Shi, Liqiao,Yin, Wenyan,Yang, Ziwen,Liang, Ying. 2016

[9]The role of apoptosis in MCLR-induced developmental toxicity in zebrafish embryos. Zeng, Cheng,Wang, Jianghua,Zhang, Guirong,Chen, Nan,Li, Guangyu,Sun, Hong,Xie, Ping,Li, Guangyu,Yan, Wei. 2014

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台