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Bioinformatics Analysis of Small RNAs in Pima (Gossypium barbadense L.)

文献类型： 外文期刊

作者： Hu, Hongtao 1 ; Yu, Dazhao 3 ; Liu, Hong 4 ;

作者机构： 1.Hubei Acad Agr Sci, Ctr Biopesticide Res, Wuhan, Hubei, Peoples R China

2.Hubei Vocat Coll Biol Sci & Technol, Dept Biol Engn, Wuhan, Hubei, Peoples R China

3.Hubei Acad Agr Sci, Inst Plant Protect & Soil Fertilizer, Wuhan, Hubei, Peoples R China

4.Hunan Univ Arts & Sci, Coll Life Sci, Changde, Hunan, Peoples R China

5.Huazhong Agr Univ, Coll Fisheries, Wuhan, Hubei, Peoples R China

期刊名称：PLOS ONE （ 2020影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2015 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Small RNAs (sRNAs) are similar to 20 to 24 nucleotide single-stranded RNAs that play crucial roles in regulation of gene expression. In plants, sRNAs are classified into microRNAs (miRNAs), repeat-associated siRNAs (ra-siRNAs), phased siRNAs (pha-siRNAs), cis and trans natural antisense transcript siRNAs (cis- and trans-nat siRNAs). Pima (Gossypium barbadense L.) is one of the most economically important fiber crops, producing the best and longest spinnable fiber. Although some miRNAs are profiled in Pima, little is known about siRNAs, the largest subclass of plant sRNAs. In order to profile these gene regulators in Pima, a comprehensive analysis of sRNAs was conducted by mining publicly available sRNA data, leading to identification of 678 miRNAs, 3,559,126 ra-siRNAs, 627 pha-siRNAs, 136,600 cis-nat siRNAs and 79,994 trans-nat siRNAs. The 678 miRNAs, belonging to 98 conserved and 402 lineage-specific families, were produced from 2,138 precursors, of which 297 arose from introns, exons, or intron/UTR-exon junctions of protein-coding genes. Ra-siRNAs were produced from various repeat loci, while most (97%) were yielded from retrotransposons, especially LTRs (long terminal repeats). The genes encoding auxin-signaling-related proteins, NBS-LRRs and transcription factors were major sources of pha-siRNAs, while two conserved TAS3 homologs were found as well. Most cis-NATs in Pima overlapped in enclosed and convergent orientations, while a few hybridized in divergent and coincided orientations. Most cis- and trans-nat siRNAs were produced from overlapping regions. Additionally, characteristics of length and the 5'-first nucleotide of each sRNA class were analyzed as well. Results in this study created a valuable molecular resource that would facilitate studies on mechanism of controlling gene expression.

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