文献类型： 外文期刊
作者： Zeng, Cheng 1 ; Sun, Hong 2 ; Xie, Ping 3 ; Wang, Jianghua 1 ; Zhang, Guirong 1 ; Chen, Nan 1 ; Yan, Wei 5 ; Li, Guangyu 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
2.Hubei Maternal & Child Hlth Hosp, Wuhan 430070, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
4.Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Peoples R China
5.Hubei Acad Agr Sci, Inst Agr Qual Stand & Testing Technol, Wuhan 430064, Peoples R China
关键词： MCLR;Zebrafish;Embryo;Apoptosis;Developmental toxicity
期刊名称：AQUATIC TOXICOLOGY （ 影响因子：4.964； 五年影响因子：5.071 ）
ISSN： 0166-445X
年卷期： 2014 年 149 卷
页码：
收录情况： SCI
摘要： We previously demonstrated that cyanobacteria-derived microcystin-leucine-arginine (MCLR) is able to induce developing toxicity, such as malformation, growth delay and also decreased heart rates in zebrafish embryos. However, the molecular mechanisms by which MCLR induces its toxicity during the development of zebrafish remain largely unknown. Here, we evaluate the role of apoptosis in MCLRinduced developmental toxicity. Zebrafish embryos were exposed to various concentrations of MCLR (0, 0.2, 0.5, 2, and 5.0 mg L-1) for 96 h, at which time reactive oxygen species (ROS) was significantly induced in the 2 and 5.0 mg L-1 MCLR exposure groups. Acridine orange (AO) staining and terminal deoxynucleotide transferase-mediated deoxy-UTP nick end labelling (TUNEL) assay showed that MCLR exposure resulted in cell apoptosis. To test the apoptotic pathway, the expression pattern of several apoptoticrelated genes was examined for the level of enzyme activity, gene and protein expression, respectively. The overall results demonstrate that MCLR induced ROS which consequently triggered apoptosis in the heart of developing zebrafish embryos. Our results also indicate that the p53-Bax-Bc1-2 pathway and the caspase-dependent apoptotic pathway play major roles in MCLR-induced apoptosis in the developing embryos. (C) 2014 Elsevier B.V. All rights reserved.
- 相关文献
作者其他论文 更多>>
-
Neurotoxicity of Combined Exposure to the Heavy Metals (Pb and As) in Zebrafish (Danio rerio)
作者：Liu, Ming;Li, Guangyu;Liu, Haoling;Zuo, Junli;Zheng, Wenting;Luan, Ning;Deng, Ping;Cui, Wenwen;Zhang, Huixian;Chen, Xin;Yao, Jingjing;Peng, Xitian;Peng, Lijun;Liu, Jiao;Yan, Wei
关键词：zebrafish; heavy metals; co-exposure; neurotoxicity; neurotransmitter; HPI axis
-
Probiotic Lactobacillus rhamnosus alleviates the neurotoxicity of microcystin-LR in zebrafish (Danio rerio) through the gut-brain axis
作者：Luan, Ning;Zuo, Junli;Niu, Qianping;Liu, Haoling;Wang, Guoao;Ma, Xufa;Qin, Jianhui;Li, Guangyu;Yan, Wei;Hung, Tien-Chieh;Wu, Qin;Deng, Ping
关键词：MCLR; Probiotic; Neurotransmitter; Gut-brain axis; HPI axis
-
Comparative Analysis of the Physicochemical Properties and Metabolites of Farinose and Crisp Lotus Roots (Nelumbo nucifera Gaertn.) with Different Geographical Origins
作者：Liu, Jiao;Yang, Jie;Wang, Jing;Peng, Xitian;Yan, Wei;Peng, Lijun;Zhou, Youxiang;Peng, Jiawen;Zhao, Liuqing
关键词：lotus root; texture; metabolites; Zeno trap; multivariate analysis
-
Facile fabrication of naphthalene-functionalized magnetic nanoparticles for efficient extraction of polycyclic aromatic hydrocarbons from environmental water and fish samples
作者：Peng, Xitian;Liu, Li;Hu, Xizhou;Yan, Wei;Zheng, Dan;Xia, Zhenzhen;Zhou, Youxiang;Xia, Hong;Peng, Lijun;Yu, Qiongwei
关键词：Polycyclic aromatic hydrocarbons; Magnetic solid-phase extraction; Naphthalene modified magnetic nanoparticles; Fish; GC-MS; MS
-
Titanium dioxide nanoparticles decreases bioconcentration of azoxystrobin in zebrafish larvae leading to the alleviation of cardiotoxicity
作者：Nie, Hongyan;Pan, Meiqi;Li, Guangyu;Chen, Juan;Yang, Qing;Hung, Tien-Chieh;Xing, Dan;Peng, Maomin;Peng, Xitian;Yan, Wei;Li, Guangyu
关键词：Titanium dioxide nanoparticles; Azoxystrobin; Cardiotoxicity; Mitochondrial dysfunction
-
Molecular mechanism of reproductive toxicity induced by beta-cypermethrin in zebrafish
作者：Lu, Juanli;Li, Guangyu;Wang, Ruiqi;Liu, Yingxin;Duan, Cunyu;Duan, Shiyun;He, Xuanyi;Huang, Zhuo;Wu, Qin;Yang, Qing;Yang, Qing;Peng, Xitian;Yan, Wei;Jiang, Jinhua
关键词：Beta-cypermethrin; Zebrafish; Reproduction; HPG axis; Molecular mechanisms
-
Mechanisms of parental co-exposure to polystyrene nanoplastics and microcystin-LR aggravated hatching inhibition of zebrafish offspring
作者：Yan, Wei;Wu, Qin;Li, Guangyu;Yan, Wei;Wu, Qin;Huo, Tangbin;Du, Xue;Yang, Qing;Hung, Tien-Chieh
关键词：Embryonic hatching inhibition; Co-exposure; Microcystins-LR; Polystyrene nanoplastics; Mechanisms
