您好，欢迎访问湖北省农业科学院 机构知识库！
湖北省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Evaluation of a real-time RT-PCR assay using minor groove binding probe for specific detection of Chinese wild-type classical swine fever virus

文献类型： 外文期刊

作者： Wen, Guoyuan 1 ; Zhang, Tao 2 ; Yang, Jun 1 ; Luo, Qingping 1 ; Liao, Yonghong 1 ; Hu, Zhibin 4 ; Zhang, Rongrong 1 ; Wan 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Wuhan 430070, Peoples R China

2.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China

3.Hubei Key Lab Anim Embryo & Mol Breeding, Wuhan 430070, Peoples R China

4.Hubei Prov Anim Dis Control Ctr, Wuhan 430064, Peoples R China

5.Chinese Acad Sci, Grad Univ, Beijing 10004

关键词： Classical swine fever virus;Real-time RT-PCR;Differential diagnosis;Wild-type strain;HCLV strain

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2011 年 176 卷 1-2 期

页码：

收录情况： SCI

摘要： A one-step real-time RT-PCR assay using a minor groove binding probe was developed for the specific detection of Chinese wild-type classical swine fever virus (CSFV). The assay detected wild-type CSFV strains representing different genotypes, but did not amplify viral RNA from the Hog Cholera Lipinized Virus (HCLV) vaccine-strain and other porcine viruses. The assay had a detection limit of 10 copies/reaction or 3.0 median tissue culture infective dose/reaction. In comparison to the sequencing nested RT-PCR assay, the sensitivity and specificity of the assay were 98.3% and 94.3%. respectively, when testing 515 veterinary samples. Wild-type CSFV RNA was detected in nasal swabs 2-4 days before detection in serum samples from pigs exposed to infection by contact. and 2-4 days prior to the onset of clinical disease. HCLV RNA remained undetectable in nasal swabs and serum samples from vaccinated pigs. In conclusion, the novel assay described in this study provides a rapid and sensitive method for differentiating between wild-type and the HCLV-strain of CSFV. It could be used for monitoring in CSF outbreak areas or as a screening method for CSFV eradication strategies. (C) 2011 Elsevier B.V. All rights reserved.

  • 相关文献

[1]A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification. Kang, Kang,Luo, Jun,Kang, Kang,Zhong, Jiasheng,Zhang, Limin,Gou, Deming,Yang, Keli,Tian, Yongxiang,Zhai, Jianxin,Zhang, Li,Song, Changxu,Gou, Christine Yuan. 2014

[2]Chimeric classical swine fever (CSF)-Japanese encephalitis (JE) viral replicon as a non-transmissible vaccine candidate against CSF and JE infections. Yang, Zhenhua,Wu, Rui,Li, Ling,Guo, Deyin,Pan, Zishu,Li, Robert W.,Xiong, Zhongliang,Zhao, Haizhong.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：湖北省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台