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Evaluation of latex agglutination inhibition reaction test for rapid detection of aflatoxin B-1

文献类型： 外文期刊

作者： Ye, Yang 3 ; Wang, Ping 3 ; Zhou, Youxiang 4 ; Chen, Fusheng 1 ; Wang, Xiaohong 1 ;

作者机构： 1.Huazhong Agr Univ, Minist Agr, Key Lab Food Safety Evaluat, Wuhan 430070, Hubei Province, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Agromicrobiol, Wuhan 430070, Hubei Province, Peoples R China

3.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei Province, Peoples R China

4.Minist Agr, Food Qual Inspect & Testing Ctr, Wuhan 430064, Hubei Province, Peoples R China

关键词： LAIRT;Latex agglutination test;CPLP;Mycotoxin;AFB(1);Immunoassay

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2011 年 22 卷 7 期

页码：

收录情况： SCI

摘要： Aflatoxin B-1 (AFB(1)), a mycotoxin mainly produced by some Aspergillus species, has been found in a wide range of agricultural products. To avoid the risk of AFB(1) consumption, many agricultural commodities, foods and feeds should be analyzed and a rapid and non-instrumental method for the detection of AFB(1) is needed. In this study, a rapid, inexpensive and user-friendly latex agglutination inhibition reaction test (LAIRT) for on site testing of AFB(1) had been established. At first, carboxylated polystyrene latex particles (CPLP) were prepared by soap-free polymerization and sensitized with aflatoxin B-1 oxime-BSA (AFB(1)-O-BSA) for the detection of AFB(1). In LAIRT, the agglutination reaction with AFB(1)O-BSA-sensitized CPLP and anti-AFB(1) antiserum mixture was inhibited by 5 ng/mL AFB(1) and the analysis time for 6 samples on one glass slide was less than 10 min. Subsequently, 10 rice and peanut samples were analyzed by LAIRT and ELISA, and the results showed that 1 rice sample and 2 peanut samples were positive and in agreement with those of ELISA. However, the results could be obtained more rapidly by LAIRT than ELISA. This easy and rapid LAIRT might be useful for screening AFB(1) of agricultural commodities, foods and feeds in the field. (C) 2011 Elsevier Ltd. All rights reserved.

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[1]Rapid detection of aflatoxin B-1 on membrane by dot-immunogold filtration assay. Ye, Yang,Mo, Zhenzhen,Cheng, Wei,Yang, Sha,Wang, Xiaohong,Chen, Fusheng,Wang, Xiaohong,Chen, Fusheng,Chen, Fusheng,Zhou, Youxiang.

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