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HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana

文献类型： 外文期刊

作者： Wang Hui 1 ; Wang Jiayuan 1 ; Wu Xiaopeng 1 ; Shen Haiyan 1 ; Luo Ying 1 ; Dai Haofu 2 ; Mei Wenli;

作者机构： 1.Chinese Acad Trop Agr Sci, Hainan Key Lab Res & Dev Nat Prod Li Folk Med, Key Lab Trop Crop Biotechnol, Minist Agr,Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China

2.Chinese Acad Trop Agr Sci, Hainan Key Lab Res & Dev Nat Prod Li Folk Med, Key Lab Trop Crop Biotechnol, Minist Agr,Inst Trop Biosci &

关键词： Flavonoid;Dragon's blood;High performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS);Dracaena cambodiana;Tissue culture

期刊名称：CHEMICAL RESEARCH IN CHINESE UNIVERSITIES （ 影响因子：1.307； 五年影响因子：0.979 ）

ISSN： 1005-9040

年卷期： 2015 年 31 卷 1 期

页码：

收录情况： SCI

摘要： Dragon's blood is a traditional medicine and used in many countries with different cultures because of its various therapeutic properties. The main bioactive constituents of dragon's blood are flavonoids, which exhibit various pharmaceutical activities, such as haemostatic, analgesic, anticoagulant, and antimicrobial activities and so on, and have attracted the attention of researchers on the development of new drugs. However, the formation process of dragon's blood in nature is very slow, which cannot meet the demand of pharmaceutical uses. Dracaena cambodiana (D. cambodiana) is one of the resource plants of dragon's blood. During the course of tissue culture of D. cambodiana, 6-benzylaminopurine was added in the medium, then red secretion was discovered in the cultured medium. Twelve compounds(1-12), including 11 flavonoids, were determined via the analysis of constituents of the cultured medium by high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS) and the results were compared with that of the standards isolated from dragon's blood. Among compounds 1-12, 4,4'-dihydroxy-2-methoxydihydrochalcone(5), 4,4'-dihydroxy-2,6-dimethoxydihydrochalcone(6) and (2S)-7,3'-dihydroxy-4'-methoxyflavane(9) were major compounds of the red secretion, with contents of 15.70, 7.10 and 57.23 mg/L, respectively. Therefore, it is promising that flavonoids from dragon's blood can be obtained from the tissue culture of its resource plants for the purpose of drug development.

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