您好,欢迎访问中国热带农业科学院 机构知识库!

Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum

文献类型: 外文期刊

作者: Qiu, Jian 1 ; Sun, Shuquan 2 ; Luo, Shiqiao 1 ; Zhang, Jichuan 2 ; Xiao, Xianzhou 1 ; Zhang, Liqun 2 ; Wang, Feng 3 ; Liu 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Biol & Genet Resources Rubber Tree, Danzhou 571737, Hainan, Peoples R China

2.Beijing Univ Chem & Technol, Coll Mat & Engn, Ctr Adv Elastomer Mat, Beijing 100029, Peoples R China

3.Shandong Linglong Tyre Co Ltd, Zhaoyuan 265406, Shandong, Peoples R China

4.Chinese Acad Trop Agr Sci, Zhanjiang Expt Stn, Zhanjiang 524013, Guangdong, Peoples R China

关键词: Anthocyanins;Natural rubber;Agrobacterium;Taraxacum brevicorniculatum;AtPAP1 genes;HPLC-MS

期刊名称:PLANT CELL REPORTS ( 影响因子:4.57; 五年影响因子:4.463 )

ISSN: 0721-7714

年卷期: 2014 年 33 卷 4 期

页码:

收录情况: SCI

摘要: This study developed a new purple coloured Taraxacum brevicorniculatum plant through genetic transformation using the Arabidopsis AtPAP1 gene, which overproduced anthocyanins in its vegetative tissues. Rubber-producing Taraxacum plants synthesise high-quality natural rubber (NR) in their roots and so are a promising alternative global source of this raw material. A major factor in its commercialization is the need for multipurpose exploitation of the whole plant. To add value to the aerial tissues, red/purple plants of the rubber-producing Taraxacum brevicorniculatum species were developed through heterologous expression of the production of anthocyanin pigment 1 (AtPAP1) transcription factor from Arabidopsis thaliana. The vegetative tissue of the transgenic plants showed an average of a 48-fold increase in total anthocyanin content over control levels, but with the exception of pigmentation, the transgenic plants were phenotypically comparable to controls and displayed similar growth vigor. Southern blot analysis confirmed that the AtPAP1 gene had been integrated into the genome of the high anthocyanin Taraxacum plants. The AtPAP1 expression levels were estimated by quantitative real-time PCR and were highly correlated with the levels of total anthocyanins in five independent transgenic lines. High levels of three cyanidin glycosides found in the purple plants were characterized by high performance liquid chromatography-mass spectrum analysis. The presence of NR was verified by NMR and infrared spectroscopy, and confirmed that NR biosynthesis had not been affected in the transgenic Taraxacum lines. In addition, other major phenylpropanoid products such as chlorogenic acid and quercetin glycosides were also enhanced in the transgenic Taraxacum. The red/purple transgenic Taraxacum lines described in this study would increase the future application of the species as a rubber-producing crop due to its additional health benefits.

  • 相关文献

[1]国产沉香HPLC-MS研究. 杨锦玲,梅文莉,董文化,王军,戴好富. 2018

[2]Efficient Sugarcane Transformation via bar Gene Selection. Wang, W. Z.,Yang, B. P.,Feng, C. L.,Wang, J. G.,Xiong, G. R.,Zhao, T. T.,Zhang, S. Z.. 2017

[3]Monetary Policy and the Prices Volatility of Natural and Synthetic Rubber in China. LIU Ruijin. 2013

[4]Study on Curing Kinetics of Natural Rubber dried by Microwave. Mei Chen,Fuquan Zhang,Yongzhou Wang,Maofang Huang. 2013

[5]Monetary Policy and the Price Volatility of Natural and Synthetic Rubber in China. Liu Ruijin. 2014

[6]Non-invasive expressions of ipt in whole plants or roots indicate cytokinins are synthesized in plant aerial parts, and coordinate with light affect on the phenotypes and the formation of anthocyanins, lignins. Guo, Jian-Chun,Hu, Xin-Wen. 2010

[7]Interactive effects of cytokinins, light, and sucrose on the phenotypes and the syntheses of anthocyanins and lignins in cytokinin overproducing transgenic Arabidopsis. Guo, JC,Hu, XW,Duan, RJ. 2005

[8]Enzymatic browning and antioxidant activities in harvested litchi fruit as influenced by apple polyphenols. Zhang, Zhengke,Qu, Hongxia,Yun, Ze,Wang, Hui,Huang, Zihui,Huang, Hua,Jiang, Yueming,Zhang, Zhengke,Huber, Donald J..

[9]Fingerprints of anthocyanins and flavonols of Vaccinium uliginosum berries from different geographical origins in the Greater Khingan Mountains and their antioxidant capacities. Su, Shang,Wang, Li-Jin,Feng, Cheng-Yong,Liu, Yang,Du, Hui,Wang, Liang-Sheng,Su, Shang,Wang, Li-Jin,Feng, Cheng-Yong,Liu, Yang,Li, Chong-Hui,Tang, Zhong-Qiu,Xu, Yan-Jun.

[10]Transcriptome-Wide Identification and Characterization of MYB Transcription Factor Genes in the Laticifer Cells of Hevea brasiliensis. Wang, Ying,Zhan, Di-Feng,Li, Hui-Liang,Guo, Dong,Zhu, Jia-Hong,Peng, Shi-Qing,Zhan, Di-Feng. 2017

[11]Effect of Peroxide Content on Morphology and Properties of Thermoplastic Vulcanizates Based on PP and NR. Wang, Rui,Peng, Zheng,Wang, Rui,Fan, Pengpeng. 2011

[12]Preparation and Characterization of Nano-crystalline cellulose/natural rubber(NCC/NR) composites. Gao, Tianming,Huang, Maofang,Xie, Ruihong,Chen, Honglian. 2013

[13]Thermal Stability and Mechanical Properties of Modified Starch/NR Composite. Li, Si-Dong,Wang, Zhi-Fen,Li, Kai-Mian,Huang, Jie,Wang, Zhi-Fen,Fu, Xin. 2010

[14]Rheological Behavior of Raw Natural Rubber Coagulated by Microorganisms. Wang, Zhifen,Luo, Wenjie,Fang, Lin,Liao, Shuangquan,Li, Lefan,Lin, Hua,Li, Sidong,He, Canzhong. 2014

[15]Preparation and Antibacterial Property Evaluation of TiO2/Nature Rubber Nanocomposites. Huang, Maofang,Ding, Aiwu,Gao, Tianming,Lv, Mingzhe,Li, Puwang. 2010

[16]Thermo-oxidative Degradation of Natural Rubber Coagulated by Microorganisms. Li, Si-Dong,Zhong, Jie-Ping,Yang, Lei,Liao, Shuang-Quan,She, Xiao-Dong,He, Can-Zhong. 2010

[17]Effect of accelerator type on dynamic properties of natural rubber vulcanizates. Gao, Tianming,Huang, Maofang,Li, Puwang,Xie, Ruihong. 2014

[18]Graft Copolymerization of Natural Rubber with Maleoyl Chitosan for Antibiosis. Li, Yongzhen,Li, Puwang,Lv, Mingzhe,Zhang, Fan. 2016

[19]Thermal stability and mechanical properties of modified starch/NR composite. Li, Si-Dong,Wang, Zhi-Fen,Huang, Jie,Wang, Zhi-Fen,Fu, Xin,Lin, Hua,She, Xiao-Dong. 2010

[20]Effect of Oyster Shell Powder Loading on the Mechanical and Thermal Properties of Natural Rubber/Oyster Shell Composites. Li, Lefan,Wang, Zhifen,Zeng, Zongqiang,Peng, Zheng,She, Xiaodong,Li, Sidong,Zhong, Jieping. 2017

作者其他论文 更多>>