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Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum

文献类型： 外文期刊

作者： Qiu, Jian 1 ; Sun, Shuquan 2 ; Luo, Shiqiao 1 ; Zhang, Jichuan 2 ; Xiao, Xianzhou 1 ; Zhang, Liqun 2 ; Wang, Feng 3 ; Liu 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Rubber Res Inst, Key Lab Biol & Genet Resources Rubber Tree, Danzhou 571737, Hainan, Peoples R China

2.Beijing Univ Chem & Technol, Coll Mat & Engn, Ctr Adv Elastomer Mat, Beijing 100029, Peoples R China

3.Shandong Linglong Tyre Co Ltd, Zhaoyuan 265406, Shandong, Peoples R China

4.Chinese Acad Trop Agr Sci, Zhanjiang Expt Stn, Zhanjiang 524013, Guangdong, Peoples R China

关键词： Anthocyanins;Natural rubber;Agrobacterium;Taraxacum brevicorniculatum;AtPAP1 genes;HPLC-MS

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2014 年 33 卷 4 期

页码：

收录情况： SCI

摘要： This study developed a new purple coloured Taraxacum brevicorniculatum plant through genetic transformation using the Arabidopsis AtPAP1 gene, which overproduced anthocyanins in its vegetative tissues. Rubber-producing Taraxacum plants synthesise high-quality natural rubber (NR) in their roots and so are a promising alternative global source of this raw material. A major factor in its commercialization is the need for multipurpose exploitation of the whole plant. To add value to the aerial tissues, red/purple plants of the rubber-producing Taraxacum brevicorniculatum species were developed through heterologous expression of the production of anthocyanin pigment 1 (AtPAP1) transcription factor from Arabidopsis thaliana. The vegetative tissue of the transgenic plants showed an average of a 48-fold increase in total anthocyanin content over control levels, but with the exception of pigmentation, the transgenic plants were phenotypically comparable to controls and displayed similar growth vigor. Southern blot analysis confirmed that the AtPAP1 gene had been integrated into the genome of the high anthocyanin Taraxacum plants. The AtPAP1 expression levels were estimated by quantitative real-time PCR and were highly correlated with the levels of total anthocyanins in five independent transgenic lines. High levels of three cyanidin glycosides found in the purple plants were characterized by high performance liquid chromatography-mass spectrum analysis. The presence of NR was verified by NMR and infrared spectroscopy, and confirmed that NR biosynthesis had not been affected in the transgenic Taraxacum lines. In addition, other major phenylpropanoid products such as chlorogenic acid and quercetin glycosides were also enhanced in the transgenic Taraxacum. The red/purple transgenic Taraxacum lines described in this study would increase the future application of the species as a rubber-producing crop due to its additional health benefits.

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