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Cloning and Analysis of Vacuolar Invertase Gene (MeVINV2) Promoter from Cassava (Manihot Esculenta Crantz)

文献类型： 外文期刊

作者： Liu, Jiao 1 ; Hu, Yan-ping 1 ; Xia, Wen-rui 1 ; Yao, Yuan 1 ; Zhou, Yang 1 ; Fu, Shao-ping 1 ; Duan, Rui-jun 2 ; Li, Rui- 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

2.Chinese Acad Trop Agr Sci, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Inst Trop Biosci & Biote

关键词： Cassava;Vacuolar invertase;Promoter;Cis-acting regulatory elements

期刊名称：MATERIALS, MACHINES AND DEVELOPMENT OF TECHNOLOGIES FOR INDUSTRIAL PRODUCTION

ISSN： 1660-9336

年卷期： 2014 年 618 卷

页码：

收录情况： SCI

摘要： Vacuolar invertases play a vital role in the progress of cassava tuber roots starch accumulation. In order to study the regulating mechanism of cassava vacuolar invertases, the promoter of cassava vacuolar invertase 2 (MeVINV2) was isolated using the PCR amplification approach, starting with a part of coding sequences. Sequencing result showed that 47 bp MeVINV2 gene CDS sequence and 1242 bp potential promoter sequence was obtained. PlantCARE analysis revealed that the MeVINV2 gene promoter contains typical eukaryotic elements CAAT box and TATA box, and also several light-responsive elements and stress-responsive elements. These cis-acting regulatory elements might be associated to the vacuolar invertase gene function of cassava starch accumulation and biological stress defense.

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[1]Cloning, 3D Modeling and Expression Analysis of Three Vacuolar Invertase Genes from Cassava (Manihot Esculenta Crantz). Yao, Yuan,Wu, Xiao-Hui,Li, Rui-Mei,Liu, Jiao,Guo, Jian-Chun,Yao, Yuan,Wu, Xiao-Hui,Geng, Meng-Ting,Hu, Xin-Wen.

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