您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

An Fe Deficiency Responsive Element with a Core Sequence of TGGCA Regulates the Expression of FEA1 in Chlamydomonas reinharditii

文献类型： 外文期刊

作者： Fei, Xiaowen 1 ; Eriksson, Mats 3 ; Yang, Jinghao 1 ; Deng, Xiaodong 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Minist Agr, Haikou 571101, Peoples R China

2.Hainan Med Coll, Dept Biochem, Haikou 571101, Peoples R China

3.Umea Univ, Umea Plant Sci Ctr, SE-90187 Umea, Sweden

关键词： Chlamydomonas reinharditii;FEA1;FeREs;iron;promoter

期刊名称：JOURNAL OF BIOCHEMISTRY （ 影响因子：3.387； 五年影响因子：3.169 ）

ISSN： 0021-924X

年卷期： 2009 年 146 卷 2 期

页码：

收录情况： SCI

摘要： Iron is essential to the unicellular green alga Chlamydomonas, but the molecular mechanism for response to iron deficiency remains largely unknown. In previous studies, we have identified FOX1 and ATX1 FEREs (Fe deficiency-responsive elements) as important regulation components of iron response in this organism. Here we present another iron regulated gene FEA1, which promoter was analysed by using a 5'- and 3'-end deletion and a scanning mutagenesis assay. The results reveal that the co-existence of -273/-188 and -118/-49 regions from transcriptional start site of FEA1 were sufficient and necessary for Fe deficiency-induced expression. Further deletion analysis indicates both -273/-253 and -103/-85 regions are essential for inducible expression. The scanning mutagenesis analysis of these regions identifies two cis-acting elements: the FeaFeRE1 at -273/-259 (CTGCGGTGGCAAAGT) and FeaFeRE2 at -106/-85 (CCGCCGCNNNTGGCACCAGCCT). Sequence comparison of FeaFeRE1 and FeaFeRE2 reveals a core sequence of TGGCA, which had been found in our previously reported Fe-deficiency-inducible gene ATX1. Moreover, we show that the promoter region of several genes, including FRE1, IRT1, ISCA, ZRT1, ZRT5, NRAMP2 and COPT1, also contains this core sequence, suggesting that at least two classes FeRE elements exist in Clamydomonas, one in FEA1 and ATX1 and others the second in FOX1, FEA2, MTP4, NRAMP3 and RBOL1.

  • 相关文献

[1]The effects of nutritional restriction on neutral lipid accumulation in Chlamydomonas and Chlorella. Deng, Xiaodong,Fei, Xiaowen,Li, Yajun,Fei, Xiaowen. 2011

[2]Titanium as a Beneficial Element for Crop Production. Lyu, Shiheng,Wei, Xiangying,Chen, Jianjun,Pan, Dongming,Lyu, Shiheng,Wei, Xiangying,Chen, Jianjun,Wang, Cun,Wang, Cun,Wang, Xiaoming. 2017

[3]Isolation of the endosperm-specific LPAAT gene promoter from coconut (Cocos nucifera L.) and its functional analysis in transgenic rice plants. Zheng, Yusheng,Wang, Zhekui,Li, Dongdong,Xu, Li,Zhou, Peng,Ye, Rongjian,Lin, Yongjun,Ye, Rongjian,Lin, Yongjun. 2010

[4]Cloning and Analysis of Vacuolar Invertase Gene (MeVINV2) Promoter from Cassava (Manihot Esculenta Crantz). Liu, Jiao,Hu, Yan-ping,Xia, Wen-rui,Yao, Yuan,Zhou, Yang,Fu, Shao-ping,Duan, Rui-jun,Li, Rui-mei,Guo, Jian-chun. 2014

[5]HbMADS4, a MADS-box Transcription Factor from Hevea brasiliensis, Negatively Regulates HbSRPP. Li, Hui-Liang,Wei, Li-Ran,Guo, Dong,Wang, Ying,Zhu, Jia-Hong,Chen, Xiong-Ting,Peng, Shi-Qing. 2016

[6]Low-Temperature- and Phosphate Deficiency-Responsive Elements Control DGTT3 Expression in Chlamydomonas reinhardtii. Fei, Xiaowen,Li, Ping,Li, Xinghan,Deng, Xiaodong. 2018

[7]Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response. Liu, Linya,Huang, Yacheng,Wu, Wenqiang,Xu, Yun,Cong, Ziwen,Xie, Jun,Xia, Wei,Huang, Dongyi,Liu, Linya,Huang, Yacheng,Huang, Xiaolong,Yang, Jianghua. 2017

[8]Promoter regulatory domain identification of cassava starch synthase IIb gene in transgenic tobacco. Guan, Zhihui,Chen, Xin,Xie, Hairong,Wang, Wenquan,Guan, Zhihui,Chen, Xin,Xie, Hairong,Wang, Wenquan.

[9]Molecular characterization of HbCZF1, a Hevea brasiliensis CCCH-type zinc finger protein that regulates hmg1. Guo, Dong,Yi, Hong-Yan,Li, Hui-Liang,Liu, Chen,Yang, Zi-Ping,Peng, Shi-Qing,Yi, Hong-Yan,Liu, Chen,Yang, Zi-Ping.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台