文献类型： 外文期刊

作者： Wu, Jian-Guo ¹ ; Peng, Wei ² ; Yi, Jun ³ ; Wu, Yan-Bin ¹ ; Chen, Ti-Qiang ⁴ ; Wong, Ka-Hing ⁵ ; Wu, Jin-Zhong ¹ ;

作者机构： 1.Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou 350108, Peoples R China

2.Third Mil Med Univ, Coll Pharm, Dept Pharmacol, Chongqing 400038, Peoples R China

3.Fujian Inst Educ, Dept Chem & Life Sci, Fuzhou 350001, Peoples R China

4.Fujian Acad Agr Sci, Inst Edible & Med Fungi, Fuzhou 350013, Peoples R China

5.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Hong Kong, Hong Kong, Peoples R China

关键词： Toona sinensis (A. Juss.) Roem;Essential oil;Sesquiterpene;Anti-Staphlococcal activity;Cytotoxicity;Apoptosis

期刊名称：JOURNAL OF ETHNOPHARMACOLOGY （ 影响因子：4.36； 五年影响因子：4.488 ）

ISSN： 0378-8741

年卷期： 2014 年 154 卷 1 期

页码：

收录情况： SCI

摘要： Ethnopharmacological relevance: Leaves of Toona sinensis (A. Juss.) Roem. (TSL), a popular vegetable in China, have anti-inflammatory, antidoting, and worm-killing effects and are used in folk medicine for the treatment of enteritis, dysentery, carbuncles, boils, and especially abdominal tumors. Our aim was to investigate the in vitro antimicrobial activity against Staphylococcus aureus and anticancer property of the essential oil from TSL (TSL-EO), especially the pro-apoptotic effect in SGC-7901. Materials and methods: TSL-EO obtained by hydrodistillation was analyzed by GC/MS and was tested in vitro against twenty clinically isolated strains of Staphylococcus aureus (SA 1-20), which were either methicillin-sensitive Staphylococcus aureus (MSSA) or methicillin-resistant Staphylococcus aureus (MRSA) and two standard strains viz. ATCC 25923 and ATCC 43300. The anticancer activity of TSL-EO was evaluated in vitro against HepG2, SGC7901, and HT29 through MIT assay. Moreover, the apoptosisinducing activity of TSL-EO in SGC7901 cells was determined by Hoechst 33324 staining and flow cytometry methods. Also, the apoptosis-related proteins viz. Bax, Bc1-2 and caspase-3 were detected by western-blotting. Results: GC-MS analysis showed that TSL-EO contained a high amount of sesquiterpenes (84.64%), including copaene (8.27%), beta-caryophyllene (10.16%), caryophyllene (13.18%) and beta-eudesmene (5.06%). TSL-EO inhibited the growth of both MSSA and MRSA, with the lowest MIC values of 0.125 and 1 mg/ml, respectively. Treatment with TSL-EO for 24 h could significantly suppress the viability of three different cancer cell lines (P <0.05). Furthermore, the apoptosis-inducing activity of TSL-EO in SGC7901 cells increased in a dose-dependent manner, potentially resulting from the up-regulated expression of Bax, caspase-3 and down-regulated expression of Bc1-2. Conclusions: TSL-EO possessed antibacterial activity against Staphylococcus aureus and significant cytotoxicity against cancer cells and particularly prominent pro-apoptotic activity in SGC7901 cells. These bioactivities were probably due to the high content of sesquiterpenes. Our results suggested that TSL-EO possessed potential health benefits and could serve as a promising natural food addictive. (C) 2014 Elsevier Ireland Ltd. All rights reserved.