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Osteoblastic activity of ethanolic extract and volatile compounds from Er-Zhi-Wan, a famous traditional Chinese herbal formula

文献类型： 外文期刊

作者： Wu, Yan-Bin 1 ; Wu, Jian-Guo 1 ; Yi, Jun 2 ; Chen, Ti-Qiang 3 ; Lin, Xiong-Hao 1 ; Wu, Jin-Zhong 1 ;

作者机构： 1.Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou 350122, Fujian, Peoples R China

2.Fujian Inst Educ, Dept Chem & Life Sci, Fuzhou 350001, Fujian, Peoples R China

3.Fujian Acad Agr Sci, Inst Edible & Med Fungi, Fuzhou 350013, Peoples R China

关键词： Er-Zhi-Wan ( EZW);volatile compounds;osteoblasts;GC-MS

期刊名称：AFRICAN JOURNAL OF PHARMACY AND PHARMACOLOGY （ 影响因子：0.839； 五年影响因子：0.875 ）

ISSN： 1996-0816

年卷期： 2012 年 6 卷 24 期

页码：

收录情况： SCI

摘要： Er-Zhi-Wan (EZW), a famous traditional Chinese formula, which is comprised of the aerial parts of Eclipta prostrate L. (EP) (Astraceae) and the fruits of Ligustrum lucidum Ait. (FLL) (Oleaceae) has been developed as a restorative formula for hundreds of years. It is widely used to prevent and treat various kidney diseases. This study aimed to investigate the effects of volatile components and ethanolic extract from EZW on the proliferation and differentiation of primary osteoblasts by the MTT method and measuring the activity of alkaline phosphatase (ALP). Both ethanolic extract and volatile components from EZW could significantly (p<0.01) stimulate the proliferation and increase the ALP activity of primary osteoblasts. The volatile components of EZW were analyzed by GC-MS. A total of 61 compounds, which were the major part (about 86.34%) of the volatiles were identified by matching mass spectra with a mass spectrum library (NIST 05.L) and retention indexes (RI) of the compounds reported on equivalent column.

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