Reverse transcription loop-mediated isothermal amplification for rapid detection of the newly emerged poultry Flavivirus
文献类型： 外文期刊
作者： Li, Zhaolong 1 ; Chen, Shaoying 1 ; Wang, Shao 1 ; Chen, Shilong 1 ; Lin, Fengqian 1 ;
作者机构： 1.Fujian Acad Agr Sci, Dept Anim Husb & Vet Med, Fuzhou 350013, Peoples R China
2.Fujian Acad Agr Sci, Dept Fujian Anim Dis Control Technol Dev Ctr, Fuzhou 350013, Peoples R China
期刊名称：FOLIA MICROBIOLOGICA （ 影响因子：2.099； 五年影响因子：2.131 ）
ISSN： 0015-5632
年卷期： 2013 年 58 卷 4 期
页码：
收录情况： SCI
摘要： Poultry Flavivirus (PF) was a recently emerged virus with high morbidity rates and mortality rates in China. It is the causative agent of egg drop syndrome at present. Development of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was the most efficient way to prevent and control the PF disease. The assay was performed at 64 degrees C for 45 min, using six specific primers that recognized eight targets of the PF E gene. The RT-LAMP assay, compared to conventional reverse transcription polymerase chain reaction, has 100-fold-greater sensitivity, with a detection limit of 1 x 10(-3) copies per mu L RNA and no cross-reaction with poultry other viruses. The RT-LAMP assay is a valuable tool for detected PF without requiring any sophisticated equipment, and the detection has potential usefulness for clinical diagnosis in the field.
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