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Comparative transcriptome analysis of the calcium signaling and expression analysis of sodium/calcium exchanger in Aspergillus cristatus

文献类型： 外文期刊

作者： Ren, Xiu-xiu 1 ; Wang, Yuchen 3 ; Liu, YongXiang 3 ; Tan, Yumei 3 ; Ren, Chunguang 3 ; Ge, Yongyi 3 ; Liu, Zuoyi 3 ;

作者机构： 1.Guizhou Univ, Coll Agr, Guiyang, Guizhou, Peoples R China

2.Guizhou Univ Engn Sci, Coll Ecol Engn, Bijie, Peoples R China

3.Guizhou Acad Agr Sci, Jiaxiunan Rd, Guiyang 550006, Guizhou, Peoples R China

关键词： Aspergillus cristatus;Ca2+-signaling pathway;expression analysis;sodium;calcium exchanger

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

ISSN： 0233-111X

年卷期： 2018 年 58 卷 1 期

页码：

收录情况： SCI

摘要： Aspergillus cristatus develops into various stages under different Na concentrations: the sexual stage in 0.5M NaCl and asexual development stage in 3M NaCl. In order to explore whether the Ca2+ signaling pathway in A. cristatus responded to the changes in the salt stress, we analyzed the gene expression levels in A. cristatus respectively cultured in 0.5M NaCl and 3M NaCl. According to the BLAST analysis results, we identified 25 Ca2+-signaling proteins in A. cristatus. The expression levels of most genes involved in the Ca2+-signaling pathway in A. cristatus cultured in different salt concentrations showed significant differences, indicating that the Ca2+ signaling pathway was involved in the response to the changes in the salt stress. In yeasts, only calcium ion influx proteins were reported to be involved in the response to the changes in the salt stress. So far, the protein for the exchanger of calcium/sodium ions has not been reported. Therefore, we obtained the sodium/calcium exchanger (termed NCX) proteins from the KEGG Database. The ncx gene of A. cristatus was cloned and characterized. The full length of ncx gene is 3055bp, including a 2994-bp open reading frame encoding 994 amino acids. The expression levels of ncx in the sexual development stage and asexual development stage were respectively approximate to 8.94 times and approximate to 2.57 times of that in the hyphal formation stage. Therefore, we suggested that ncx gene was up-regulated to resist the sodium stress. The study results provide the basis for further exploring the Ca2+-signaling mechanism and ion exchanger mechanism.

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[1]Comparative genomic and transcriptomic analyses of the Fuzhuan brick tea-fermentation fungus Aspergillus cristatus. Ge, Yongyi,Ren, Xiuxiu,Ge, Yongyi,Wang, Yuchen,Liu, YongXiang,Tan, Yumei,Ren, Xiuxiu,Liu, Zuoyi,Ge, Yongyi,Wang, Yuchen,Liu, YongXiang,Tan, Yumei,Ren, Xiuxiu,Zhang, Xinyu,Hyde, Kevin D.,Liu, Yongfeng,Liu, Zuoyi. 2016

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