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A Comparative Proteomic Analysis of Pinellia ternata Leaves Exposed to Heat Stress

文献类型： 外文期刊

作者： Zhu, Yunhao 1 ; Zhu, Guosheng 2 ; Guo, Qiaosheng 1 ; Zhu, Zaibiao 1 ; Wang, Changlin 1 ; Liu, Zuoyi 3 ;

作者机构： 1.Nanjing Agr Univ, Inst Chinese Med Mat, Nanjing 210095, Jiangsu, Peoples R China

2.Guizhou Acad Agricaltural Sci, Inst Morden Chinese Med Mat, Guiyang 550006, Peoples R China

3.Guizhou Acad Agr Sci, Key Lab Agr Biotechnol, Guiyang 550006, Peoples R China

关键词： proteomics;quantitative real-time PCR;heat stress;Pinellia ternata

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2013 年 14 卷 10 期

页码：

收录情况： SCI

摘要： Pinellia ternata is an important traditional Chinese medicinal plant. The growth of P. ternata is sensitive to high temperatures. To gain a better understanding of heat stress responses in P. ternata, we performed a comparative proteomic analysis. P. ternata seedlings were subjected to a temperature of 38 degrees C and samples were collected 24 h after treatment. Increased relative ion leakage and lipid peroxidation suggested that oxidative stress was frequently generated in rice leaves exposed to high temperature. Two-dimensional electrophoresis (2-DE) was used to analyze heat-responsive proteins. More than 600 protein spots were reproducibly detected on each gel; of these spots, 20 were up-regulated, and 7 were down-regulated. A total of 24 proteins and protein species were successfully identified by MALDI-TOF/TOF MS. These proteins and protein species were found to be primarily small heat shock proteins (58%) as well as proteins involved in RNA processing (17%), photosynthesis (13%), chlorophyll biosynthetic processes (4%), protein degradation (4%) and defense (4%). Using 2-DE Western blot analysis, we confirmed the identities of the cytosolic class II small heat shock protein (sHSPs-CII) identified by MS. The expression levels of four different proteins [cytosolic class I small heat shock protein (sHSPs-CI), sHSPs-CII, mitochondrial small heat shock protein (sHSPs-MIT), glycine-rich RNA-binding protein (GRP)] were analyzed at the transcriptional level by quantitative real-time PCR. The mRNA levels of three sHSPs correlated with the corresponding protein levels. However, GRP was down-regulated at the beginning of heat stress but then increased substantially to reach a peak after 24 h of heat stress. Our study provides valuable new insight into the responses of P. ternata to heat stress.

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