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Genetic diversity in Vicia amoena (Fabaceae) germplasm resource in China using SRAP and ISSR markers

文献类型： 外文期刊

作者： Liu, Ying 1 ; Zhang, Ju-ming 1 ; Wang, Xian-guo 2 ; Liu, Fang 3 ; Shen, Zhong-bao 4 ;

作者机构： 1.South China Agr Univ, Coll Agr, Guangzhou 510642, Guangdong, Peoples R China

2.China Agr Univ, Coll Anim Sci & Technol, Beijing 100193, Peoples R China

3.Natl Herbage Germplasm Conservat Ctr China, Beijing 100193, Peoples R China

4.Heilongjiang Acad Agr Sci, Inst Grassland, Harbin 150086, Heilongjiang, Peoples R China

关键词： Vicia amoena;Genetic diversity;SRAP markers;ISSR markers

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

ISSN： 0305-1978

年卷期： 2013 年 51 卷

页码：

收录情况： SCI

摘要： The genetic diversity in 17 germplasms of Vicia amoena L from north China was analyzed using SRAP and ISSR markers. Three hundred and sixty-eight (94.11%) polymorphic bands (211 and 157 obtained from 20 pairs of SRAP and ISSR primers, respectively) were scored. Although SRAP was more effective than ISSR markers with higher PIC, RP and larger variation range of genetic distance, both the markers were useful for assessing V amoena genetic diversity. Cluster analysis showed that the 17 germplasms were clustered into 5 groups. The results of principal coordinate analysis supported UPGMA clustering. The germplasms from source areas where the annual average temperature ranged from -1.0 to 5 degrees C exhibited the highest level of genetic diversity with the highest PPI, I and H. These results have important implications in genome mapping, breeding purposes, and germ-plasm conservation. (C) 2013 Elsevier Ltd. All rights reserved.

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