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HSF IS THE MOST IMPORTANT TRANSCRIPTIONAL FACTOR FOR PORCINE MC4R PROMOTER

文献类型： 外文期刊

作者： Zhang, D. J. 1 ; Liu, D. 1 ; Wang, L. 1 ; Zhang, X. H. 1 ; Bie, S. 1 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China

关键词： Firefly luciferase gene vector;MC4R;Pig;Promoter

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.282； 五年影响因子：1.702 ）

ISSN： 1049-5398

年卷期： 2012 年 23 卷 2 期

页码：

收录情况： SCI

摘要： The melanocortin-4 receptor (MC4R) is involved in feed intake regulation. It is significantly associated with growth and fatness traits in most breeds and crosses. To understand the essential transcriptional regions of the porcine MC4R promoter, eleven primer pairs were designed to amplify different segment lengths of the MC4R promoter. The PCR products were then ligated along with the Firefly luciferase reporter gene into the PGL3-basic vector. The results showed that a 90 bp fragment could contain the essential regions for control of transcription. Further research found that a short sequence "AGAAAGAAG'' (the recognition site of transcription factor HSF) was the most important sequence for supporting activity of the pig MC4R promoter.

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