您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effect of Cycloheximide on the Development of Parthenogenetic Activation and Cloned Porcine Embryos

文献类型： 外文期刊

作者： Ma, Hong 1 ; Fu, Bo 1 ; Li, Zhongqiu 1 ; Liu, Di 1 ; Zhao, Jinfeng 2 ; Fang, Qingchang 2 ; Ren, Liang 2 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Anim Husb Res Inst, Haerbin, Peoples R China

2.Northeast Agr Univ, Anim Sci & Technol, Haerbin, Peoples R China

关键词： cycloheximide;parthenogenetic activation;somatic cell nuclear transfer

期刊名称：ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 2

ISSN：

年卷期： 2011 年

页码：

收录情况： SCI

摘要： The purpose of this study was to investigate the effect of cycloheximide on the development of parthenogenetic activation and somatic cell nuclear transfered porcine embryos. porcine oocytes (44h after IVM) treated using electrical activation combinations of cycloheximide or cytochalsin B or 6-dimethylaminipurine were evaluated to determine the development rates of parthenogenetic activation porcine embryos (Group 1).Pretreatment with cycloheximide before electrical activation combinations of different reagents were evaluated to determine the development rates of parthenogenetic activation porcine embryos (Group 2).The groups were comprised of the treated using electrical activation combinations of different reagents were evaluated to determine the development rates of cloned porcine embryos (Group 3).The results show that the cycloheximide exhibited significantly (P<0.05) lower rates of parthenogenetic activation cleavage rates and of blastocyst rates, and longer time of parthenogenetic activation. However, Pre-treatment with cycloheximide significantly increased parthenogenetic activation cleavage rates and of blastocyst rates. Treated using electrical activation combinations of cycloheximide had the highest blastocyst yield of cloned porcine embryos.

  • 相关文献

[1]Supplementation with glucose in PZM-3 medium improve the in vitro development of porcine transgenic cloned embryos. Fu, Bo,Liu, Di,Ma, Jian-zhang,An, Tie-zhu,Ren, Liang,Guo, Yun-yun,Kang, Ya,Zhao, Cheng-yue,Ma, Hong,Li, Zhong-qiu,Wang, Wen-tao,Bai, Jing. 2011

[2]Effect of Pyruvate and Lactic Acid on the Development of Porcine Embryos. Fu Bo,Llu Di,Ma Hong,Li Zhong-qiu,Wang Liang,Bai Jing. 2012

[3]Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei. Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-Hua,Wang, Liang,Li, Zhong-Qiu,Peng, Fu-Gang,Liu, Di,Bai, Jing.

[4]Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos. Liu Di,Ma Hong,Wang Liang,Guo Zhen-hua,Li Zhong-qiu,Fu Bo,Liu Di,Fang Qing-chang,Ren Liang.

[5]Using a nano-flare probe to detect RNA in live donor cells prior to somatic cell nuclear transfer. Fu, Bo,Ma, Jian-zhang,An, Tie-zhu,Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-hua,Ren, Liang,Liu, Di,Yang, Xiu-qin,Zhu, Meng,Bai, Jing.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台