文献类型： 外文期刊

作者： Qiu, Xuewen ¹ ; Kuang, Huiyun ² ; Zeng, Chuntao ¹ ; Li, Dan ¹ ; Yang, Youxin ¹ ; Gan, Yudi ¹ ; Fan, Shuying ¹ ; Wu, Caijun ¹ ;

作者机构： 1.Jiangxi Agr Univ, Coll Agron, Nanchang 330045, Jiangxi, Peoples R China

2.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai, Peoples R China

关键词： Vigna unguiculata; polyploidy; phenotype and physiological characteristics; transcriptome sequencing; differentially expressed gene (DEG)

期刊名称：CIENCIA RURAL （ 影响因子：0.8； 五年影响因子：0.9 ）

ISSN： 0103-8478

年卷期： 2024 年 54 卷 8 期

页码：

收录情况： SCI

摘要： This study investigated the differences in gene expression profiles of diploid and autotetraploid in cowpea, and provided theoretical basis for screening key genes of differential expression and ploidy breeding. The phenotypes and contents of chlorophyll, soluble sugar and soluble protein of diploid and autotetraploid of cowpea were compared and transcriptome sequencing was performed. The autotetraploid leaves of cowpea were thicker and darker green than diploid leaves, and the contents of chlorophyll, soluble sugar and soluble protein in leaves were higher. A total of 2678 differentially expressed genes (DEGs) were analyzed in the diploid and autotetraploid of cowpea. Among them, there were 421 genes with higher expression of tetraploid than diploid, and 2257 genes with lower expression of tetraploid than diploid. All 2678 DEGs were annotated into the Gene Ontology (GO) functional library. The DEGs were mainly concentrated in metabolism and cell composition. Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analysis showed that cowpea diploid and autotetraploid have significant differences in flavonoid biosynthesis, degradation of other glycan, phenylpropane biosynthesis, starch sucrose metabolism, keratin, amber and wax biosynthesis, circadian rhythm and plant pathways.