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An arginine-to-histidine mutation in flavanone-3-hydroxylase results in pink strawberry fruits

文献类型： 外文期刊

作者： Xu, Pengbo 1 ; Li, Xinyu 1 ; Fan, Junmiao 1 ; Tian, Shuhua 2 ; Cao, Minghao 1 ; Lin, Anqi 1 ; Gao, Qinhua 4 ; Xiao, Kun 1 ; Wang, Chong 1 ; Kuang, Huiyun 6 ; Lian, Hongli 1 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai Collaborat Innovat Ctr Agri Seeds, Shanghai 200240, Peoples R China

2.Peking Univ, Sch Adv Agr Sci, Beijing 100871, Peoples R China

3.Lishui Univ, Dept Ecol, Lishui 323000, Peoples R China

4.Shanghai Acad Agr Sci, Forestry & Fruit Tree Res Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China

5.Hebei Normal Univ Sci & Technol, Coll Hort Sci, Qinhuangdao 066004, Peoples R China

6.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201403, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）

ISSN： 0032-0889

年卷期： 2023 年 193 卷 3 期

页码：

收录情况： SCI

摘要： Fruit color is a very important external commodity factor for consumers. Compared to the most typical red octoploid strawberry (Fragaria x ananassa), the pink strawberry often sells for a more expensive price and has a higher economic benefit due to its outstanding color. However, few studies have examined the molecular basis of pink-colored strawberry fruit. Through an EMS mutagenesis of woodland strawberry (Fragaria vesca), we identified a mutant with pink fruits and green petioles. Bulked-segregant analysis sequencing analysis and gene function verification confirmed that the responsible mutation resides in a gene encoding flavanone-3-hydroxylase (F3H) in the anthocyanin synthesis pathway. This nonsynonymous mutation results in an arginine-to-histidine change at position 130 of F3H. Molecular docking experiments showed that the arginine-to-histidine mutation results in a reduction of intermolecular force-hydrogen bonding between the F3H protein and its substrates. Enzymatic experiments showed a greatly reduced ability of the mutated F3H protein to catalyze the conversion of the substrates and hence a blockage of the anthocyanin synthesis pathway. The discovery of a key residue in the F3H gene controlling anthocyanin synthesis provides a clear target of modification for the molecular breeding of strawberry varieties with pink-colored fruits, which may be of great commercial value.

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