文献类型： 外文期刊

作者： Kumar, Suthar Teerath ¹ ; Zhang, Yunpeng ¹ ; Zhang, Qi ¹ ; Azeem, Riaz Muhammad ³ ; Jing, Zhang ⁴ ; Pan, Li ¹ ; Sun, Wu-Sheng ³ ; Zhao, Yuan ¹ ; Zhang, Shu-Min ¹ ;

作者机构： 1.Jilin Agr Univ, Coll Anim Sci & Technol, Key Lab Anim Prod Prod Qual & Secur, Minist Educ, Changchun 130118, Peoples R China

2.Jilin Acad Agr Sci, Inst Anim Husb & Vet, Changchun 136100, Peoples R China

3.Jilin Agr Univ, Engn Res Ctr Microecol Vaccines Drugs Major Anim D, Jilin Prov Key Lab Anim Microecol & Hlth Breeding, Coll Vet Med,Jilin Prov Engn Res Ctr Anim Probiot,, 2888 Xincheng St, Changchun 130118, Peoples R China

4.Acad Natl Food & Strateg Reserv Adm, Key Lab Grain & Oil Biotechnol, 11 Baiwanzhuang Ave, Beijing 100037, Peoples R China

关键词： Fat deposition; Gut microbiomes; Gut-muscle axis; Meat quality; Metabolites; Pig breeds

期刊名称：BMC MICROBIOLOGY （ 影响因子：4.2； 五年影响因子：4.8 ）

ISSN： 1471-2180

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundGut microbiota ferment non-digestible substances to produce metabolites that accumulate in muscle and influence host metabolism. However, the regulatory mechanisms connecting gut microbiota, metabolites, and fat deposition across pig breeds remain unclear. This study explores the gut-muscle axis regulating fat deposition and meat quality in Chinese Songliao Black Pig (SBP) and Large White x Landrace Pigs (LWLDP). Digesta samples from the ileum, cecum, and rectum of both breeds were analyzed using 16 S rRNA sequencing for microbiome profiling and ultra-high-performance liquid chromatography (UHPLC) for metabolomics. Multi-omics data, including microbiota and metabolite profiles were integrated with our previously published data of transcriptomics and metabolomics insights into fat deposition in the longissimus dorsi (LD) muscle using the MixOmics DIABLO method.ResultsMicrobiome analysis revealed that Fibrobacter, Unidentified_Peptostreptococcaceae, Sutterella, and Unidentifed_Rickettsiales were enriched in SBP, while Ruminococcus, Corynebacterium, and Streptococcaceae in LWLDP. Metabolomic analysis indicated that SBP was enriched in fatty acid biosynthesis pathways, including linoleic acid, alpha-linolenic acid, and arachidonic acid, whereas LWLDP was associated with insulin signaling, starch and sucrose metabolism. Integrated analysis identified Peptostreptococcaceae and Rickettsiales in SBP, along with metabolites phosphatidylcholine (PC(22:4)), N-acylethanolamine (NAE(20:4)), and lysophosphatidylcholine (LysoPC(24:1)) were correlated with key genes (EIF4E, MSTN, PPARGC1A, NR4A3, and SOCS1) regulating fat deposition. In LWLDP, Corynebacterium and Streptococcaceae were linked to the PPP1R3B gene, which is involved in glycogen metabolism, as well as metabolites 2-methyl-3-hydroxybutyric acid and 5-keto-gluconic acid, suggesting a shift toward glycolysis over lipolysis.ConclusionThis study concluded that cecum-associated microbes in LWLDP may enhance carbohydrate metabolism, leading to reduced fat deposition, whereas rectum-associated microbes in SBP contribute to docosahexaenoic acid (DHA) biosynthesis, thereby improving meat quality. These findings highlight gut microbiota-derived metabolites as potential biomarkers for optimizing meat production and livestock breeding strategies.