CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens
文献类型： 外文期刊
作者： Jiao, Zhe 1 ; Yang, Jialing 1 ; Long, Xiaojuan 2 ; Lu, Yingfang 2 ; Guo, Zongning 3 ; Peng, Yonglin 4 ; Huang, Xuelin 3 ; Yin, Yi 5 ; Song, Chao 6 ; Zhang, Pengfei 8 ;
作者机构： 1.Dongguan Univ Technol, Sch Environm & Civil Engn, Dongguan, Peoples R China
2.Guangdong Dongguan Ecol & Environm Monitoring Stn, Dongguan, Peoples R China
3.Huangpu Customs Dist Technol Ctr, Dongguan, Peoples R China
4.Pinete Zhongshan Biotechnol Co Ltd, Zhongshan, Peoples R China
5.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Peoples R China
6.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi, Jiangsu, Peoples R China
7.Minist Agr & Rural Affairs, Key Lab Control Qual & Safety Aquat Prod, Beijing, Peoples R China
8.Chinese Acad Sci, Shenzhen Engn Lab Nanomed & Nanoformulat, CAS Key Lab Hlth Informat,Shenzhen Inst Adv Techn, Guangdong Key Lab Nanomed,Inst Biomed & Biotechno, Shenzhen, Peoples R China
关键词： CRISPR-Cas12a; pathogenic microorganisms; aggregation-induced emission (AIE); visual detection; logic gate
期刊名称：FRONTIERS IN CHEMISTRY （ 2021影响因子：5.545； 五年影响因子：5.869 ）
ISSN： 2296-2646
年卷期： 2022 年 9 卷
收录情况： SCI
摘要： Here, we developed a rapid, visual and double-checked Logic Gate detection platform for detection of pathogenic microorganisms by aggregation-induced emission luminogens (AIEgens) in combination with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas). DNA light-up AIEgens (1,1,2,2-tetrakis[4-(2-bromo-ethoxy) phenyl]ethene, TTAPE) was non-emissive but the emission was turned on in the presence of large amount of DNA produced by recombinase polymerase amplification (RPA). When CRISPR/Cas12a was added, all long-stranded DNA were cut leading to the emission quenched. Thus, a method that can directly observe the emission changes with the naked eye has been successfully constructed. The detection is speedy within only 20 min, and has strong specificity to the target. The result can be judged by Logic Gate. Only when the output signal is (1,0), does it represent the presence of pathogenic microorganisms in the test object. Finally, the method was applied to the detect pathogenic microorganisms in environmental water samples, which proved that this method has high selectivity, specificity and applicability for the detection of pathogenic microorganisms in environmental water samples.
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