Complete Genome Sequencing Analysis of Deinococcus wulumuqiensis R12, an Extremely Radiation-Resistant Strain
文献类型： 外文期刊
作者： Dai, Zijie 1 ; Zhang, Zhidong 4 ; Zhu, Liying 2 ; Zhu, Zhengming 1 ; Jiang, Ling 1 ;
作者机构： 1.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211816, Peoples R China
2.Nanjing Tech Univ, Coll Chem & Mol Engn, Nanjing 211816, Peoples R China
3.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing 211816, Peoples R China
4.Xinjiang Acad Agr Sci, Xinjiang Key Lab Special Environm Microbiol, Inst Appl Microbiol, Urumqi 830091, Xinjiang, Peoples R China
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.343； 五年影响因子：2.408 ）
ISSN： 0343-8651
年卷期： 2022 年 79 卷 10 期
页码：
收录情况： SCI
摘要： Genome sequencing was performed by the PacBio RS II platform and Illumina HiSeq 4000 platform to discover the metabolic profile of the Deinococcus wulumuqiensis R12, which was isolated from radiation-contaminated soils in Xinjiang Uygur Autonomous Region of northwest China. The genome of 3.5 Mbp comprises one circular chromosome and four circular plasmids with 3679 genes and a GC content of 66.97%. A total of 41 new transcriptional factors were identified using the DeepTFactor tool. Genomic analysis revealed the presence of genes for homologous recombination repair, which suggested high recombination efficiency in R12. Three Type I and one Type II RM systems, two CRISPR arrays, and one Cas-Type IC protein were found, allowing the development of endogenous CRISPR-Cas gene-editing tools. Additionally, we found that R12 has a broad spectrum of substrate utilization, which was validated by physiological experiments. Genes involved in the carotenoid biosynthesis pathway and the antioxidative system were also identified. Overall, the comprehensive description of the genome of R12 will facilitate the additional exploitation of this strain as a versatile cell factory for biotechnological applications.
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