Virulent Phage vB_EfaS_WH1 Removes Enterococcus faecalis Biofilm and Inhibits Its Growth on the Surface of Chicken Meat
文献类型： 外文期刊
作者： Jin, Xinxin 1 ; Sun, Xiuxiu 2 ; Wang, Zui 1 ; Dou, Junfeng 1 ; Lin, Zhengdan 2 ; Lu, Qin 1 ; Zhang, Tengfei 1 ; Wen, Guoyuan 1 ; Shao, Huabin 1 ; Cheng, Guofu 2 ; Luo, Qingping 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr & Rural Affairs, Wuhan 430064, Peoples R China
2.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China
3.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Prov Key Lab Anim Pathogen Microbiol, Wuhan 430064, Peoples R China
关键词： Enterococcus faecalis; bacteriophage; biofilm; genome; biocontrol agent
期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）
ISSN：
年卷期： 2023 年 15 卷 5 期
页码：
收录情况： SCI
摘要： Enterococcus faecalis is a potential animal and human pathogen. Improper use of antibiotics encourages resistance. Bacteriophages and their derivatives are promising for treating drug-resistant bacterial infections. In this study, phylogenetic and electron microscopy analyses of phage vB_EfaS_WH1 (WH1) isolated from chicken feces revealed it to be a novel phage in the family Siphoviridae. WH1 showed good pH stability (4-11), temperature tolerance (4-60 degrees C), and broad E. faecalis host range (60% of isolates). Genome sequencing revealed a 56,357 bp double-stranded DNA genome with a G+C content of 39.21%. WH1 effectively destroyed E. faecalis EF01 biofilms, even at low concentrations. When WH1 was applied at 1 x 10(5) to 1 x 10(9) PFU/g to chicken breast samples stored at 4 ffiC, surface growing E. faecalis were appreciably eradicated after 24 h. The phage WH1 showed good antibacterial activity, which could be used as a potential biocontrol agent to reduce the formation of E. faecalis biofilm, and could also be used as an alternative for the control of E. faecalis in chicken products.
- 相关文献
作者其他论文 更多>>
-
Research note: Prevalence and genetic characteristics of pathogenic E. coli isolates from domestic pigeons in central China
作者：Wang, Juan;Lu, Qin;Yao, Lun;Zhang, Wenting;Hu, Qiao;Guo, Yunqing;Wen, Guoyuan;Shao, Huabin;Luo, Qingping;Zhang, Tengfei;Wen, Guoyuan;Luo, Qingping
关键词：Pathogenic E. coli; Pigeons; Serotypes; Antibiotic resistance
-
Identification of SepF in Streptococcus suis involving cell division
作者：Gao, Ting;Zhu, Jiajia;Liu, Wei;Yang, Keli;Zhang, Tengfei;Yuan, Fangyan;Liu, Zewen;Guo, Rui;Li, Chang;Wu, Qiong;Tian, Yongxiang;Zhou, Danna;Li, Tingting;Zheng, Linlin;Zhou, Rui;Zheng, Linlin;Chen, Mo
关键词：
Streptococcus suis ; Cell division; Septum; Protein-protein interaction; Drug target -
Construction and bacteriostatic effect analyses of a recombinant thermostable Newcastle disease virus expressing cecropin AD
作者：Yao, Lun;Ren, Xiangfei;Yang, Junjie;Zeng, Zhe;Shang, Yu;Feng, Helong;Jin, Mengyun;Xiao, Qianni;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Ren, Xiangfei;Yang, Junjie;Hu, Sishun;Yao, Lun;Ren, Xiangfei;Yang, Junjie;Zeng, Zhe;Shang, Yu;Feng, Helong;Jin, Mengyun;Xiao, Qianni;Shao, Huabin;Luo, Qingping;Wen, Guoyuan;Zhou, Ping;Zeng, Chi;Luo, Qingping
关键词：Newcastle disease virus; Cecropin AD; Staphylococcus aureus; Wound healing
-
Addressing unexpected bacterial RNA safety concerns of E. coli produced influenza NP through CpG loaded mutant
作者：Chen, Cen;Li, Mengling;Guo, Aili;Guo, Pengju;Zhang, Wanpo;Gu, Changqin;Zhou, Hongbo;Tao, Pan;Chen, Cen;Li, Mengling;Guo, Aili;Guo, Pengju;Zhou, Hongbo;Tao, Pan;Wen, Guoyuan
关键词：
-
Genetic and pathogenic characteristics of a novel recombinant GI-19 infectious bronchitis virus strain isolated from northeastern China
作者：Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Zeng, Zhe;Yao, Lun;Feng, Helong;Wang, Zichen;Jiang, Liren;Wang, Haojie;Shang, Yu;Wang, Hongcai;Shao, Huabin;Wen, Guoyuan;Luo, Qingping;Wen, Guoyuan;Luo, Qingping;Feng, Helong;Zhou, Chengli
关键词：Infectious bronchitis virus; GI-19; Evolutionary; Recombination; Pathogenicity; Antigenic variation
-
Research Note: Isolation and genomic characterization of Avibacterium paragallinarum from the Hubei Province, China
作者：Guo, Yunqing;Yin, Mengyu;Zhang, Tengfei;Zhang, Wenting;Lu, Qin;Hu, Qiao;Luo, Qingping;Zhai, Xinguo;Luo, Qingping
关键词：Avibacterium paragallinarum; Antimicrobial susceptibility; Virulence; Genomic characterization
-
The impacts of animal agriculture on One Health-Bacterial zoonosis, antimicrobial resistance, and beyond
作者：Zhang, Tengfei;Zhang, Wenting;Shang, Yu;Luo, Qingping;Wen, Guoyuan;Nickerson, Rhea;Cheng, Zhenyu;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Peng, Xitian;Zhou, Youxiang;Luo, Qingping
关键词：Zoonosis; Antimicrobial resistance
