Virulent Phage vB_EfaS_WH1 Removes Enterococcus faecalis Biofilm and Inhibits Its Growth on the Surface of Chicken Meat
文献类型： 外文期刊
作者： Jin, Xinxin 1 ; Sun, Xiuxiu 2 ; Wang, Zui 1 ; Dou, Junfeng 1 ; Lin, Zhengdan 2 ; Lu, Qin 1 ; Zhang, Tengfei 1 ; Wen, Guoyuan 1 ; Shao, Huabin 1 ; Cheng, Guofu 2 ; Luo, Qingping 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr & Rural Affairs, Wuhan 430064, Peoples R China
2.Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China
3.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Hubei Prov Key Lab Anim Pathogen Microbiol, Wuhan 430064, Peoples R China
关键词： Enterococcus faecalis; bacteriophage; biofilm; genome; biocontrol agent
期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）
ISSN：
年卷期： 2023 年 15 卷 5 期
页码：
收录情况： SCI
摘要： Enterococcus faecalis is a potential animal and human pathogen. Improper use of antibiotics encourages resistance. Bacteriophages and their derivatives are promising for treating drug-resistant bacterial infections. In this study, phylogenetic and electron microscopy analyses of phage vB_EfaS_WH1 (WH1) isolated from chicken feces revealed it to be a novel phage in the family Siphoviridae. WH1 showed good pH stability (4-11), temperature tolerance (4-60 degrees C), and broad E. faecalis host range (60% of isolates). Genome sequencing revealed a 56,357 bp double-stranded DNA genome with a G+C content of 39.21%. WH1 effectively destroyed E. faecalis EF01 biofilms, even at low concentrations. When WH1 was applied at 1 x 10(5) to 1 x 10(9) PFU/g to chicken breast samples stored at 4 ffiC, surface growing E. faecalis were appreciably eradicated after 24 h. The phage WH1 showed good antibacterial activity, which could be used as a potential biocontrol agent to reduce the formation of E. faecalis biofilm, and could also be used as an alternative for the control of E. faecalis in chicken products.
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