New Hydrolase from Aeromicrobium sp. HA for the Biodegradation of Zearalenone: Identification, Mechanism, and Application
文献类型： 外文期刊
作者： Hu, Junqiang 1 ; Wang, Gang 1 ; Hou, Mingxuan 1 ; Du, Shilong 1 ; Han, Jun 1 ; Yu, Yangguang 1 ; Gao, Hongxia 1 ; He, Dan 1 ; Shi, Jianrong 1 ; Lee, Yin-Won 4 ; Mohamed, Sherif Ramzy 5 ; Dawood, Dawood H. 6 ; Hong, Qing 2 ; Liu, Xin 1 ; Xu, Jianhong 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Minist Agr & Rural Affairs, Minist Agr & Rural Affairs, Inst Food Safety & Nutr,Minist Sci & Technol,Jiang, Nanjing 210014, Peoples R China
2.Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China
3.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China
4.Seoul Natl Univ, Sch Agr Biotechnol, Seoul 08826, South Korea
5.Natl Res Ctr, Food Toxicol & Contaminants Dept, Food Ind & Nutr Res Inst, Giza 12411, Egypt
6.Mansoura Univ, Fac Agr, Dept Agr Chem, Mansoura 35516, Egypt
关键词： zearalenone; biodegradation; Aeromicrobium; hydrolase; estrogenic toxicity
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）
ISSN： 0021-8561
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Zearalenone (ZEN) is an estrogenic mycotoxin most frequently found in cereals that can cause reproductive disorders in livestock and pose a severe threat to animal husbandry. In this study, we isolated a ZEN-degrading Aeromicrobium strain from soil and found that ZenH, a hydrolase, is responsible for the hydrolysis of ZEN through comparative proteomics and biochemical studies. ZenH exhibited the highest similarity with lactone hydrolase ZHD607 from Phialophora americana at 21.52%. ZenH displayed maximal enzymatic activity at pH 7.0 and 55 degrees C with a Michaelis constant of 12.64 mu M. The catalytic triad of ZenH was identified as S117-D142-H292 by molecular docking and site-directed mutagenesis. ZenH catalyzed the hydrolysis of ZEN to a novel metabolite, (S,E)-4-hydroxy-2-(10-hydroxy-6-oxoundec-1-en-1-yl)-7-oxabicyclo[4.2.0]octa-1,3,5-trien-8-one, which exhibited significantly lower estrogenic toxicity than ZEN. This study illustrates a novel ZEN-degrading enzyme and reveals a new degradation product. Furthermore, the enzyme showed good potential for detoxifying ZEN during food processing.
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