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Cloning, Expression, and Functional Characterization of FUT1, a Key Gene for Histo-Blood Group Antigens Synthesis in Crassostrea gigas

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文献类型：外文期刊

作者： Gui, Binbin ¹ ; Yao, Lin ² ; Qu, Meng ² ; Zhang, Weiran ² ; Li, Mingyu ² ; Jiang, Yanhua ² ; Wang, Lianzhu ¹ ;

作者机构： 1.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Testing & Evaluat Aquat Prod Safety & Qual, Qingdao 266071, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

4.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

5.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

关键词： Crassostrea gigas; norovirus; HBGAs; gene expression; CgFUT1

期刊名称：CURRENT ISSUES IN MOLECULAR BIOLOGY （影响因子：3.1；五年影响因子：3.3 ）

ISSN： 1467-3037

年卷期： 2023 年 45 卷 5 期

页码：

收录情况： SCI

摘要： Histo-blood group antigens (HBGAs) comprise a family of cell-surface carbohydrates that are considered norovirus-specific binding receptors or ligands. HBGA-like molecules have also been detected in oysters as common norovirus carriers, although the pathway involved in the synthesis of these molecules in oysters has yet to be elucidated. We isolated and identified a key gene involved in the synthesis of HBGA-like molecules, FUT1, from Crassostrea gigas, named CgFUT1. Real-time quantitative polymerase chain reaction analysis showed that CgFUT1 mRNA was expressed in the mantle, gill, muscle, labellum, and hepatopancreatic tissues of C. gigas, with the hepatopancreas exhibiting the highest expression level. A recombinant CgFUT1 protein with a molecular mass of 38.0 kDa was expressed in Escherichia coli using a prokaryotic expression vector. A eukaryotic expression plasmid was constructed and transfected into Chinese hamster ovary (CHO) cells. The expression of CgFUT1 and membrane localization of type H-2 HBGA-like molecules in CHO cells were detected using Western blotting and cellular immunofluorescence, respectively. This study indicated that CgFUT1, expressed in C. gigas tissues, can synthesize type H-2 HBGA-like molecules. This finding provides a new perspective for analyzing the source and synthetic pathway of HBGA-like molecules in oysters.

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