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The Holin-Endolysin Lysis System of the OP2-Like Phage X2 Infecting Xanthomonas oryzae pv. oryzae

文献类型： 外文期刊

作者： Wu, Zhifeng 1 ; Zhang, Yang 1 ; Xu, Xinyang 1 ; Ahmed, Temoor 1 ; Yang, Yong 2 ; Loh, Belinda 3 ; Leptihn, Sebastian 3 ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310058, Peoples R China

2.Zhejiang Univ, Inst Biotechnol, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Virol & Biotechnol, Key Lab Managing Biot & Chem Threats Qual & Safet, Hangzhou 310021, Peoples R China

4.Zhejiang Univ, Univ Edinburgh Inst, Hangzhou 314400, Peoples R China; Ningbo Univ, Inst Plant Virol, Ningbo 315211, Peoples R China

关键词： transmembrane domain; endolysin; holin; morphological change; lysis; phage

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN：

年卷期： 2021 年 13 卷 10 期

页码：

收录情况： SCI

摘要： Most endolysins of dsDNA phages are exported by a holin-dependent mechanism, while in some cases endolysins are exported via a holin-independent mechanism. However, it is still unclear whether the same endolysins can be exported by both holin-dependent and holin-independent mechanisms. This study investigated the lysis system of OP2-like phage X2 infecting Xanthomonas oryzae pv. oryzae, causing devastating bacterial leaf blight disease in rice. Based on bioinformatics and protein biochemistry methods, we show that phage X2 employs the classic "holin-endolysin " lysis system. The endolysin acts on the cell envelope and exhibits antibacterial effects in vitro, while the holin facilitates the release of the protein into the periplasm. We also characterized the role of the transmembrane domain (TMD) in the translocation of the endolysin across the inner membrane. We found that the TMD facilitated the translocation of the endolysin via the Sec secretion system. The holin increases the efficiency of protein release, leading to faster and more efficient lysis. Interestingly, in E. coli, the expression of either holin or endolysin with TMDs resulted in the formation of long rod shaped cells. We conclude that the TMD of X2-Lys plays a dual role: One is the transmembrane transport while the other is the inhibition of cell division, resulting in larger cells and thus in a higher number of released viruses per cell.

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