文献类型： 外文期刊
作者： He, Mengqian 1 ; Sun, Lingwei 1 ; Xu, Jiehuan 1 ; Wu, Caifeng 1 ; Zhang, Shushan 1 ; Gao, Jun 1 ; Zhang, Defu 1 ; Gan, Yeqing 4 ; Bian, Yi 4 ; Wei, Jinliang 4 ; Zhang, Weijian 5 ; Zhang, Wengang 5 ; Han, Xuejun 3 ; Dai, Jianjun 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai Municipal Key Lab Agrigenet & Breeding, Shanghai 201106, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Livestock & Poultry Resources Pig Evaluat, Shanghai 201106, Peoples R China
3.Shanghai Engn Res Ctr Breeding Pig, Shanghai 201106, Peoples R China
4.Shanghai Jiading Municipal Ctr Dis Control & Preve, Shanghai 201899, Peoples R China
5.Shanghai Municipal Ctr Dis Control & Prevent, Shanghai 200051, Peoples R China
关键词： semen cryopreservation; dry ice; semen transportation; boar
期刊名称：ANIMALS （ 影响因子：3.0； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2024 年 14 卷 10 期
页码：
收录情况： SCI
摘要： To address the safety problems posed by the transportation of boar semen using LN, this study was conducted on the short-term storage of frozen boar semen in dry ice (-79 degrees C). Boar semen frozen in LN was transferred to dry ice, kept for 1 day, 3 days, 5 days, 7 days, or 8 days, and then moved back to LN. The quality of frozen semen stored in LN or dry ice was determined to evaluate the feasibility of short-distance transportation with dry ice. The results showed that 60 degrees C for 8 s was the best condition for thawing frozen semen stored in dry ice. No significant differences in spermatozoa motility, plasma membrane integrity, or acrosome integrity were observed in semen after short-term storage in dry ice compared to LN (p > 0.05). There were no significant changes in antioxidant properties between storage groups either (p > 0.05). In conclusion, dry ice could be used as a cold source for the short-term transportation of frozen boar semen for at least 7 days, without affecting sperm motility, morphological integrity, or antioxidant indices.
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