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Duck CD8(+) T Cell Response to H5N1 Highly Pathogenic Avian Influenza Virus Infection In Vivo and In Vitro

文献类型： 外文期刊

作者： Dai, Manman 1 ; Sun, Hui 1 ; Zhao, Li 1 ; Wu, Qingxin 1 ; You, Bowen 1 ; Xu, Fengxiang 1 ; Liao, Jiayu 1 ; Zhu, Sufang 1 ; Li, Ziwei 1 ; Yao, Yongxiu 2 ; Nair, Venugopal 2 ; Liao, Ming 1 ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Guangdong Prov Key Lab Zoonosis Prevent & Control, Natl & Reg Joint Engn Lab Medicament Zoonosis Pre, Guangzhou, Peoples R China

2.Pirbright Inst, Guildford, Surrey, England

3.UK China Ctr Excellence Res Avian Dis, Guildford, Surrey, England

4.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou, Peoples R China

期刊名称：JOURNAL OF IMMUNOLOGY （ 影响因子：5.426； 五年影响因子：6.178 ）

ISSN： 0022-1767

年卷期： 2022 年 209 卷 5 期

页码：

收录情况： SCI

摘要： Domestic ducks are the important host for H5N1 highly pathogenic avian influenza virus (HPAIV) infection and epidemiology, but little is known about the duck T cell response to H5N1 AIV infection. In infection experiments of mallard ducks, we detected significantly increased CD8(+) cells and augmented expression of cytotoxicity-associated genes, including granzyme A and IFN-gamma, in PBMCs from 5 to 9 d postinfection when the virus shedding was clearly decreased, which suggested the importance of the duck cytotoxic T cell response in eliminating H5N1 infection in vivo. Intriguingly, we found that a CD8(high+) population of PBMCs was clearly upregulated in infected ducks from 7 to 9 d postinfection compared with uninfected ducks. Next, we used Smart-Seq2 technology to investigate the heterogeneity and transcriptional differences of the duck CD8(+) cells. Thus, CD8(high+) cells were likely to be more responsive to H5N1 AIV infection, based on the high level of expression of genes involved in T cell responses, activation, and proliferation, including MALT1, ITK, LCK, CD3E, CD247, CFLAR, IL-18R1, and IL-18RAP. More importantly, we have also successfully cultured H5N1 AIV-specific duck T cells in vitro, to our knowledge, for the first time, and demonstrated that the CD8(high+) population was increased with the duck T cell activation and response in vitro, which was consistent with results in vivo. Thus, the duck CD8(high+ )cells represent a potentially effective immune response to H5N1 AIV infection in vivo and in vitro. These findings provide novel insights and direction for developing effective H5N1 AIV vaccines.

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