文献类型： 外文期刊
作者： Shao, Bo-Zong 1 ; Liu, Meng-Zhen 1 ; Zhu, Dan-Ni 1 ; Yan, Hui 1 ; Ke, Ping 1 ; Wei, Wei 2 ; Han, Ting 1 ; Liu, Chong 1 ;
作者机构： 1.Second Mil Med Univ, Naval Med Univ, Dept Pharm, Shanghai 200433, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
关键词： beta-arrestin-1; Atherosclerosis; Autophagy; Macrophage; Inflammation
期刊名称：INTERNATIONAL IMMUNOPHARMACOLOGY （ 影响因子：5.6； 五年影响因子：5.6 ）
ISSN： 1567-5769
年卷期： 2023 年 125 卷
页码：
收录情况： SCI
摘要： Autophagy in atherosclerotic plaque macrophage contributes to the alleviation of atherosclerosis through the promotion of lipid metabolism. beta-arrestins are multifunctional proteins participating various kinds of cellular signaling pathways. Here we aimed to determine the role of beta-arrestin-1, an important member of beta-arrestin family, in atherosclerosis, and whether autophagy was involved in this process. ApoE(-/-)beta-arrestin-1(fl/fl)LysM-Cre mice were created through bone marrow transplantation for the atherosclerosis model with conditional myeloid knocking out beta-arrestin-1. Bone marrow-derived macrophages (BMDMs) were used for the in vitro studies. Oil red O staining was used to detect the lesional area. F4/80, Masson trichrome and picro-Sirius red staining were applied for the determination of plaque stability. Real-time PCR was used for the detection of levels of lipid metabolism-related receptors. Electron microscopy and tandem fluorescent mRFP-GFP-LC3 plasmid was applied to test autophagy level. We found that beta-arrestin-1 was highly increased in expression in plaque macrophage on the occurrence of atherosclerosis. Conditional myeloid knocking out beta-arrestin-1 largely promotes plaque for-mation and vulnerability. In murine macrophage with lipid loading, knocking down beta-arrestin-1 enhanced foam cell formation and levels of plasma and cellular cholesterol, while overexpressing beta-arrestin-1 led to the opposite effects. The alleviative effects induced by macrophage beta-arrestin-1 in atherosclerosis were involved in autophagy, based on the reduction of autophagy level with the knocking down of macrophage beta-arrestin-1 and administration of autophagy inhibitors which largely attenuated the decreasing effect on foam cell formation. Our results demonstrated for the first time that macrophage beta-arrestin-1 protected against atherosclerosis through the induction of autophagy.
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