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Supplementation with glucose in PZM-3 medium improve the in vitro development of porcine transgenic cloned embryos

文献类型： 会议论文

第一作者： Fu, Bo

作者： Fu, Bo 1 ; Liu, Di 2 ; Ma, Jian-zhang; An, Tie-zhu; Ren, Liang; Guo, Yun-yun; Kang, Ya; Zhao, Cheng-yue; Ma, Hong; Li, Zhong-qiu; Wang, Wen-tao; Bai, Jing;

作者机构： 1.Heilongjiang Acamedy of Agricultural Sciences Postdoctoral Programme

2.Northeast Forestry University Postdoctoral Programme, Harbin 150086, China

关键词： EGFP;Energy substrate;Glucose;Lactate;Pyruvate;Somatic cell nuclear transfer

会议名称： IEEE International Symposium on IT in Medicine and Education

主办单位：

页码： 728-732

摘要： The efficiency of producing porcine transgenic cloned embryos is still low due to lower developmental competence compared to in vivo-fertilized/cultured embryos. This phenomenon may correlates with poor culture condition. Energy substrate in culture medium play an important role in optimizing culture condition. This study pertain to investigate the effect of replacing pyruvate and lactate with glucose in PZM-3 medium at the rest part culture period on porcine embryonic development after SCNT. Porcine adult fibroblasts cells were transfected with pEGFP-C1 vector. Then transfected cells were used as donor cells for producing re-constructed embryos. Results have shown that supplement of glucose up to 5mM concentration in PZM-3 at the rest part of culture period improved the development of transgenic cloned embryos. Moreover, supplement of glucose(5mM) as energy substrate in PZM-3 at 48h of culture was optimal time. However, with regard to the percentage of EGFP-positive blastocyst, there was no significant difference between treatments. In conclusion, replacing pyruvate and lactate with glucose in PZM-3 medium at the rest part of culture period was beneficial to the development of transgenic cloned embryos and had no effect on transgene expression.

分类号： TP39

  • 相关文献

[1]Effect of Pyruvate and Lactic Acid on the Development of Porcine Embryos. Fu Bo,Llu Di,Ma Hong,Li Zhong-qiu,Wang Liang,Bai Jing. 2012

[2]Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos. Liu Di,Ma Hong,Wang Liang,Guo Zhen-hua,Li Zhong-qiu,Fu Bo,Liu Di,Fang Qing-chang,Ren Liang.

[3]Effect of Cycloheximide on the Development of Parthenogenetic Activation and Cloned Porcine Embryos. Ma, Hong,Fu, Bo,Li, Zhongqiu,Liu, Di,Zhao, Jinfeng,Fang, Qingchang,Ren, Liang. 2011

[4]Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei. Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-Hua,Wang, Liang,Li, Zhong-Qiu,Peng, Fu-Gang,Liu, Di,Bai, Jing.

[5]Using a nano-flare probe to detect RNA in live donor cells prior to somatic cell nuclear transfer. Fu, Bo,Ma, Jian-zhang,An, Tie-zhu,Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-hua,Ren, Liang,Liu, Di,Yang, Xiu-qin,Zhu, Meng,Bai, Jing.

[6]Effects of ultrasonic pretreatment and glycosylation on functional properties of casein grafted with glucose. Bi, Weiwei,Ge, Wei,Li, Xiaodong,Du, Lingling,Wang, Haixia,Qu, Xiuwei,Bi, Weiwei,Zhao, Guixing,Ge, Wei,Li, Xiaodong,Du, Lingling,Wang, Haixia,Qu, Xiuwei,Li, Xiaodong. 2017

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