文献类型： 会议论文

第一作者： Karen Mercier

作者： Karen Mercier ¹ ; Pierre-Emmanuel Baur; Yannick Nizet; Philippe Dulieu; Chiraz Frydman;

作者机构： 1.HORIBA Scientific, Avenue de la Vauve, Passage Jobin Yvon, Palaiseau, F-91120 France

会议名称： Protein Engineering Summit.

主办单位：

页码： 112-112

摘要： A monoclonal antibody developed at Synabs by a new hybridoma technology for guinea pig Mab was rescued and produced by Diaclone. That monoclonal antibody is highly specific to a steroid hormone (undisclosed) of 290 Da and was analysed by the SPRi technology thanks to the XelPleX system. The antibody was immobilized in different conditions (different immobilization buffers) on a single biochip functionalized with a dextran-based chemistry using the SPRi-Continuous Flow Microspotter (SPRi-CFM). A comparison with an immobilization using a contact spotting (SPRi-Arrayer) on a 2D surface chemistry was also performed. A limit of detection of the hormone of 1.5 nM (0.45 ng/mL) was determined for both method of immobilization. The specificity of the antibody was also checked by flowing an analogue hormone, at the same concentrations. Finally, an affinity of 0.2 nM of the antibody for the hormone was calculated in the EzFit software. 144 different sensorgrams were generated in a single biochip. The results obtained by SPRi are identical to the results obtained by classical ELISA technique usually used in the validation process of Diaclone. Thanks to the array-based format of the SPRi sensor chips, the XelPleX system can easily extend its performances to multiple interactions and integrate quickly biomolecules production processes such as the validation of rescued recombinant monoclonal antibodies production.

分类号： q81