Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

文献类型： 外文期刊

第一作者： Qiao Mengfan

作者： Qiao Mengfan;Wang Lixia;Li Chongyang;Qiao Jun;Meng Qingling;Qiao Mengfan;Lei Ying;Ren Yan;Cai Kuojun;Zhang Jinsheng;Zhang Zaichao;Yu Weiwei;Peng Yelong;Cai Xuepeng

作者机构：

关键词： Ehrlichia canis; genetic characteristics; gp36; pet dog; Rhipicephalus sanguineus sensu lato

期刊名称：VETERINARY WORLD （ 影响因子：1.6； 五年影响因子：2.0 ）

ISSN： 0972-8988

年卷期： 2020 年 13 卷 5 期

页码：

收录情况： SCI

摘要： Background and Aim: As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials and Methods: A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene. Results: The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic analysis of E. canis gp36 protein, these E. canis strains in different geographical regions of the world can be divided into Genogroup I and Genogroup II. Among them, the Xinjiang epidemic strain XJ-6 and 533, 36, 1055, Kasur1, and Jake strains were clustered into subgroup 1.1 of Genogroup I, while the XJ-2, XJ-21, and XJ-35 strains and the TWN1, TWN4, CM180, and CM196 strains were closely related and belonged to subgroup 2.2 of Genogroup II, displaying high genetic diversity. Conclusion: This is the first study focusing on the molecular epidemiology of E. canis infection in pet dogs, which revealed that E. canis infection had been occurred in Xinjiang, China. More importantly, this study confirmed that the substantial variability in immunoreactive protein gp36 from E. canis strains circulating in pet dogs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序