Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep

文献类型： 外文期刊

第一作者： Liu Tianli

作者： Liu Tianli;Wang Xifeng;Tian Zhenzhong;Wang Lixia;Qiao Jun;Meng Qingling;Gong Shasha;Chen Ying;Zhang Xingxing;Cai Xuepeng

作者机构：

关键词： multi-epitope; fusion protein; serodiagnostic; antigen; cystic echinococcosis; indirect ELISA; sheep

期刊名称：KOREAN JOURNAL OF PARASITOLOGY （ 影响因子：1.341； 五年影响因子：1.669 ）

ISSN： 0023-4001

年卷期： 2019 年 57 卷 1 期

页码：

收录情况： SCI

摘要： Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. Coll and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

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