Antimicrobial resistance, virulence gene profile and molecular typing of Staphylococcus aureus isolates from dairy cows in Xinjiang Province, northwest China

文献类型： 外文期刊

第一作者： Meng Dan

作者： Meng Dan;Wu Yehui;Meng Qingling;Qiao Jun;Zhang Xingxing;Ma Shuai;Cai Kuojun;Zhang Jinsheng;Cheng Zibing;Zhang Zaichao;Cai Xuepeng

作者机构： Shihezi Univ, Coll Anim Sci & Technol, North St 4, Shihezi 832003, Xinjiang, Peoples R China;Ctr Anim Dis Prevent & Control, Urumqi 830000, Xinjiang, Peoples R China;Ctr Anim Dis Prevent & Control, Tacheng 834700, Xinjiang, Peoples R China;Ctr Anim Dis Prevent & Control, Changji 831100, Xinjiang, Peoples R China;Chinese Acad Agr Sci, State Key Lab Vet Etiol Biol, Lanzhou Vet Res Inst, Lanzhou 730046, Gansu, Peoples R China

关键词： Staphylococcus aureus; Dairy cows; Antimicrobial resistance; Virulence gene; Molecular typing

期刊名称：JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE （ 2020影响因子：4.035； 五年影响因子：3.611 ）

ISSN： 2213-7165

年卷期： 2019 年 16 卷

页码：

收录情况： SCI

摘要： Objectives: Staphylococcus aureus (SA) is a major pathogen causing dairy cow mastitis and endometritis. Recently, animal-derived SA strains pose a serious public-health threat. However, little is known about antimicrobial resistance and virulence factors of SA isolated from dairy cows in Xinjiang, China. In this study, antimicrobial resistance, virulence gene profiles and genotypes of SA from clinical mastitis and endometritis in dairy cows were investigated. Methods: A total of 337 clinical samples (186 milk samples from clinical mastitis cases and 151 endometritis swab samples) were collected from 15 large-scale dairy farms and were screened for SA. All SA isolates were subjected to antimicrobial susceptibility testing, detection of virulence genes and molecular typing. Results: A total of 155 SA strains were isolated; 22 (14.2%) were methicillin-resistant S. aureus (MRSA). Resistance of MRSA isolates was significantly higher than that of methicillin-susceptible S. aureus (MSSA). The percentage of virulence genes varied between MSSA and MRSA. The strains could be divided into two SCCmec types (I and IVa), three agr types (I, II and III) and four spa types (t779, t2883, t13751 and t1939). MLST identified 14 sequence types, among which ST1 and ST9 had relatively high detection rates. Conclusions: These findings revealed that ST9-t1939-agrl was the main genotype of MSSA, whilst ST1SCCmecl-t1939-agrl was the main genotype of MRSA from dairy cows. More significantly, a novel ST (STX) was identified for the first time. The majority of SA strains from dairy cows were multidrug-resistant and carried multiple virulence genes, posing a potential public-health risk. (C) 2018 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

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