Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus

文献类型： 外文期刊

第一作者： Liao, Liqin

作者： Liao, Liqin;Chen, Weiguo;Zhang, Xiangyu;Yan, Yiming;Xie, Zi;Li, Hongxing;Lin, Wencheng;Ma, Jingyun;Zhang, Xinheng;Xie, Qingmei;Liao, Liqin;Chen, Weiguo;Yan, Yiming;Xie, Zi;Li, Hongxing;Lin, Wencheng;Ma, Jingyun;Zhang, Xinheng;Xie, Qingmei;Zhang, Huanmin;Li, Aijun;Liao, Liqin;Chen, Weiguo;Zhang, Xiangyu;Yan, Yiming;Xie, Zi;Li, Hongxing;Lin, Wencheng;Ma, Jingyun;Zhang, Xinheng;Xie, Qingmei;Chen, Weiguo;Lin, Wencheng;Ma, Jingyun;Xie, Qingmei

作者机构：

关键词： Semen extracellular vesicles (SE); Subgroup J avian leukosis virus (ALV-J); Infection; Vertical transmission

期刊名称：VIROLOGICA SINICA （ 影响因子：6.947； 五年影响因子：5.997 ）

ISSN： 1674-0769

年卷期： 2022 年 37 卷 2 期

页码：

收录情况： SCI

摘要： Subgroup J avian leukosis virus (ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is responsible for the congenital infection of progeny from generation to generation. Increasing evidence has suggested that extracellular vesicles (EVs) derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses. However, the role of EVs in infection and transmission of ALV-J remains obscure. In the present study, semen extracellular vesicles (SE) were isolated and purified from ALV-J-infected rooster seminal plasma (SE-ALV-J), which was shown to contain ALV-J genomic RNA and partial viral proteins, as determined by RNA sequencing, reverse transcription-quantitative PCR and Western blotting. Furthermore, SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection. More importantly, artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens, and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks. Taken together, the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission, enhancing our understanding on mechanisms underlying ALV-J transmission.

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