Metabolic engineering and mechanical investigation of enhanced plant autoluminescence

文献类型： 外文期刊

第一作者： Zheng, Peng

作者： Zheng, Peng;Ge, Jieyu;Ji, Jiayi;Zhong, Jingling;Chen, Hongyu;Luo, Daren;Li, Wei;Bi, Bo;Ma, Yongjun;Tong, Wanghui;Han, Leiqin;Pan, Ronghui;Fan, Pengxiang;Du, Hao;Zheng, Peng;Li, Wei;Bi, Bo;Pan, Ronghui;Du, Hao;Zhong, Jingling;Ma, Siqi;Zhang, Yuqi;Wu, Jianping;Zhao, Yanqiu;Lu, Mengzhu

作者机构：

关键词： Plant autoluminescence; FBP; energy; metabolic engineering; abiotic stress

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2023 年 21 卷 8 期

页码：

收录情况： SCI

摘要： The fungal bioluminescence pathway (FBP) was identified from glowing fungi, which releases self-sustained visible green luminescence. However, weak bioluminescence limits the potential application of the bioluminescence system. Here, we screened and characterized a C3'H1 (4-coumaroyl shikimate/quinate 3' -hydroxylase) gene from Brassica napus, which efficiently converts p-coumaroyl shikimate to caffeic acid and hispidin. Simultaneous expression of BnC3' H1 and NPGA (null-pigment mutant in A. nidulans) produces more caffeic acid and hispidin as the natural precursor of luciferin and significantly intensifies the original fungal bioluminescence pathway (oFBP). Thus, we successfully created enhanced FBP (eFBP) plants emitting 3 9 1011 photons/min/cm(2), sufficient to illuminate its surroundings and visualize words clearly in the dark. The glowing plants provide sustainable and bio-renewable illumination for the naked eyes, and manifest distinct responses to diverse environmental conditions via caffeic acid biosynthesis pathway. Importantly, we revealed that the biosynthesis of caffeic acid and hispidin in eFBP plants derived from the sugar pathway, and the inhibitors of the energy production system significantly reduced the luminescence signal rapidly from eFBP plants, suggesting that the FBP system coupled with the luciferin metabolic flux functions in an energy-driven way. These findings lay the groundwork for genetically creating stronger eFBP plants and developing more powerful biological tools with the FBP system.

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