Intronic sequences of the silkworm strains of Bombyx mori (Lepidoptera : Bombycidae): High variability and potential for strain identification

文献类型： 外文期刊

第一作者： Hong, Mee Yeon

作者： Hong, Mee Yeon;Han, Yeon Soo;Kim, Iksoo;Kim, Kee Young;Kang, Pil Don;Choi, Kwang Ho;Hwang, Jae Sam;Ryu, Kang Sun;Lee, Eun Mee;Lee, In Hee;Gui, Zhong Zheng;Jin, Byung Rae

作者机构：

关键词： genome;amino acid sequence;phylogeny;nucleotide sequence;strain characteristic;strain identification;intronic sequence;GenBank sequence numbers

期刊名称：EUROPEAN JOURNAL OF ENTOMOLOGY （ 影响因子：1.225； 五年影响因子：1.548 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： We sequenced nine introns of 25 silkworm (Bombyx mori L.) strains, assuming that the introns are particularly prone to mutation. Mean sequence divergence and maximum sequence divergence in each intronic sequence among 25 silkworm strains ranged from 0.81% (3.8 nucleotides) similar to 9.15% (85.2 nucleotides) and 1.2% (seven nucleotides) similar to 39.3% (366 nucleotides), respectively. The degree of sequence divergence in some introns is very variable, suggesting the potential of using intronic sequences for strain identification. In particular, some introns were highly promising and convenient strain markers due to the presence of a large indels (e.g., 403 bp and 329 bp) in only a limited number of strains. Phylogenetic analysis using the individual or the nine concatenated intronic sequences showed no clustering on the basis of known strain characteristics. This may further indicate the potential of the intronic sequences for the identification of silkworm strains.

分类号： Q96

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